医药导报2018，Vol.37Issue(3)：356-360,5.DOI:10.3870/j.issn.1004-0781.2018.03.020
超高效液相色谱-串联质谱法同时测定抗鼻炎中成药及保健品中19种化学药物
Simultaneous Determination of 19 Chemical Drugs in Traditional Chinese Medicines and Health Products for Treating Rhinitis by UPLC-MS/MS
张诚贤 1陈晓平 1杨红霞 1陈褚建 1张璐 1许韡金1
作者信息
- 1. 浙江省湖州市食品药品检验研究院,湖州 313000
- 折叠
摘要
Abstract
Objective To establish a UPLC-MS/MS method for simultaneous determination of 19 chemical drugs in Traditional Chinese Medicines and health products for treating rhinitis. Methods Separation was performed on Waters ACQU-ITY UPLC BEH-C18column(2.1 mm×100 mm,1.7 μm) with 0.1% formic acid acetonitrile solution and 0.1% formic acid aque-ous solution as the mobile phase by gradient elution.The flow rate was 0.3 mL·min-1.The column temperature was set at 35 ℃. The detection was performed by the positive ion electrospray ionization (ESI+) under multiple reactions monitoring (MRM) mode. Results The linear relationships of 19 chemical drugs were good in respective ranges with correlation coefficients higher than 0.995.The average recoveries of the low,medium,and high level were in the range of 84.9%-111.1%,and the RSDs were less than 5.2%.The limits of detection (LOD) and the limits of quantitation (LOQ) were in the range of 0.03-0.78 ng·mL-1 and 0.13-1.82 ng·mL-1,respectively. Conclusion The method is convenient,rapid,accurate,and sensitive,which can be used for the determination of chemical drugs added illegally in traditional Chinese medicines and health products for treating rhini-tis.关键词
抗鼻炎中成药/保健品/超高效液相色谱-串联质谱法Key words
Anti-rhinitis Traditional Chinese Medicines/Health products/UPLC-MS/MS分类
医药卫生引用本文复制引用
张诚贤,陈晓平,杨红霞,陈褚建,张璐,许韡金..超高效液相色谱-串联质谱法同时测定抗鼻炎中成药及保健品中19种化学药物[J].医药导报,2018,37(3):356-360,5.
