中国动脉硬化杂志2018，Vol.26Issue(1)：19-24,6.
人剪切修复基因D介导ox-LDL促进HUVEC凋亡作用
Hxeroderma pigmentosum mediates the effect of ox-LDL on promoting the apoptosis of HUVEC
摘要
Abstract
Aim To explore whether the hxeroderma pigmentosum D(XPD) could mediate the apoptosis of human umbilical vein endothelial cells (HUVEC) promoted by oxidized low density lipoprotein (ox-LDL).Methods The model of apoptosis of HUVEC was established by ox-LDL.XPD-siRNA was transfected into HUVEC with liposome,followed by treatment with ox-LDL.This experiment was divided into six groups:blank control group;negative control siRNA group;XPD-siRNA group;ox-LDL group;ox-LDL+negative control siRNA group;ox-LDL+XPD-siRNA group.Cell vitality was detected by MTT;Cell apoptosis rate and cell cycle were assessed with flow cytometry;The expressions of the XPD,Bax and Bcl-2 were detected by RT-PCR and Western blot.Results The optimal concentration of ox-LDL to establish the model of apoptosis of HUVEC was 100 mg/L.Compared with negative control siRNA group,the cell apoptosis rate of XPD-siRNA group was significantly decreased (P＜0.05),the survival rate was significantly increased (P＜ 0.01),the cell number in G0/G1 phase decreased (P＜0.05),while increased in S phase (P＜0.05),the expressions of XPD and Bax were declined (all P＜0.05),while the expression of Bcl-2 was elevated (P＜0.05);Compared with blank control group,the cell apoptosis rate of ox-LDL group increased significantly (P＜0.01),the survival rate was decreased (P＜0.05),the cell number in G0/G1 phase increased (P＜0.05),while decreased significantly in S phase (P＜0.01),the expressions of XPD and Bax were elevated (all P＜0.05),while the expression of Bcl-2 was declined (P＜0.05).Compared with ox-LDL+negative control siRNA group,the cell apoptosis rate of ox-LDL+XPD-siRNA group was reduced (P＜0.05),the survival rate was increased (P＜0.01),the cell number in G0/G1 phase decreased (P＜0.05),while increased in S phase (P＜0.05),the expressions of XPD and Bax were declined (all P＜0.05),while the expression of Bcl-2 was elevated (P＜0.05).Conclusion XPD can mediate the effect of ox-LDL on promoting the apoptosis of HUVEC.关键词
人剪切修复基因D/RNA干扰/人脐静脉内皮细胞/氧化型低密度脂蛋白/细胞凋亡Key words
Hxeroderma pigmentosum D/RNA interference/Human umbilical vein endothelial cells/Oxidized low density lipoprotein/Cell apoptosis分类
医药卫生引用本文复制引用
俞菊梅,胡利琳,丁颖,元文峰,李菊香,孙国芳..人剪切修复基因D介导ox-LDL促进HUVEC凋亡作用[J].中国动脉硬化杂志,2018,26(1):19-24,6.基金项目
国家自然科学基金项目(81300348) （81300348）
江西省青年科学基金资助项目(20132BAB215015) （20132BAB215015）
江西省卫生厅科技计划项目(20143084) （20143084）
