中国动脉硬化杂志2018，Vol.26Issue(1)：25-28,4.
脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制
The combined mechanism of fatty acid translocase FAT/CD36 and long chain fatty acid
摘要
Abstract
Aim To investigate the binding mechanism of fatty acid translocase FAT/CD36 and long chain fatty acid.Methods ELISA was used to analyze the intensity mechanism of the purity of FAT/CD36 binding with different fatty acid,molecular simulation docking was used to further verify the binding mechanism and analyze its possible binding sites.Results ELISA proved that the recombinant protein FAT/CD36 with different types of long chain fatty acids showed specific binding,binding values for oleic acid＞palmitic acid＞palmitoleic acid＞myristic acid＞stearic acid.Molecular simulation docking further confirmed that FAT/CD36 receptor protein mainly combines with oleic acid,palmitic acid and palmitoleic acid.And the binding site was located in the extracellular structure of the FAT/CD36 receptor protein Arg183-Lys-Gly-Lys-Arg-Asn-Leu-Ser-Tyr238 region consisting of the active pocket.Conclusion FAT/CD36,as the main receptor of long chain fatty acid transport,may transport oleic acid,palmitic acid and palmitoleic acid.关键词
脂肪酸移位酶/长链脂肪酸/分子模拟对接Key words
FAT/CD36/Long chain fatty acid/Molecular simulation docking分类
生物科学引用本文复制引用
薄俊霞,李敬达,刘庆平..脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制[J].中国动脉硬化杂志,2018,26(1):25-28,4.基金项目
国家自然科学基金项目资助(81673494) （81673494）
