中国动脉硬化杂志2018，Vol.26Issue(1)：29-34,6.
褪黑素在诱发肠系膜动脉舒张过程中对BKCa通道的直接作用机制
The direct mechanism of melatonin on the BKCa channel in its inducing relaxation of mesenteric arteries
摘要
Abstract
Ain To explore the direct mechanism of melatonin (MT) on the large-conductance calciumactivated potassium channel (BKCa channel) in its inducing relaxation of mesenteric arteries.Methods Eight-weekold male Wistar rats were selected,the mesenteric arteries were removed.The mesenteric artery tension was measured by microvessel tension test,and the gating characteristics of BKCa channel in vascular smooth muscle cells were examined by single channel patch clamp technique.Results (1) MT had no significant effect on vascular tension,but it caused dose-dependent vascular relaxation in mesenteric arteries which were induced by norepinephrine (NE).The relaxation could be inhibited by luzindole (Luz) and iberiotoxin(IbTX),and the inhibition of IbTX was more intense.(2) The results of cell-attached patch clamp recordings revealed that MT significantly increased the open probability of BKcs channel and the average opening time,significantly reduced the average closing time;Luz significantly inhibited the activation of BKca channel.(3) The results of inside-out patch clamp recordings showed that MT increased the open probability and the average opening time of BKCa channel and decreased the average closing time significantly.Conclusion MT activates the BKca channels of smooth muscle cells in mesenteric arterier directly or indirectly through the receptor,which may be one of the important mechanisms underlying the inducing of MT on mesenteric arteries.关键词
褪黑素/血管舒张/大电导钙激活钾通道Key words
Melatonin/Vasodilatation/BKCa channels分类
医药卫生引用本文复制引用
李珊珊,吴迎..褪黑素在诱发肠系膜动脉舒张过程中对BKCa通道的直接作用机制[J].中国动脉硬化杂志,2018,26(1):29-34,6.基金项目
北京市自然科学基金(5172023) （5172023）
