中国动脉硬化杂志2018，Vol.26Issue(1)：35-40,6.
苦瓜蛋白通过FXR/miR-23b-3p/HNF4α途径抑制载脂蛋白(a)表达
Momordicin 28 inhibits expression of apolipoprotein (a) by FXR/miR-23b-3p/HNF4α pathway
摘要
Abstract
Ain To study the effect of momordicin 28 (MD28) on the expression of apolipoprotein (a) in HepG2 ceils and explore its mechanism.Methods The cell viability of HepG2 ceils treated with MD28 was determined by MTT assay.MD28 was purified by centrifugation,ultrafiltration,dialysis,cation exchange chromatography and reversed-phase high performance liquid chromatography.The mRNA and protein expression levels of apolipoprotein(a),farnesyl ester X receptor (FXR) and hepatocyte nuclear factor 4t (HNF4ct) were detected by qRT-PCR and Western blot respectively,qRT-PCR was used to detect the expression level of hsa-miR-23b-3p,and small interfering RNA transfection was used to silence FXR.Results Compared with the control group,the cell viability of HepG2 cells treated with MD28 at 0.1,0.2,0.4,0.8 and 1.6 g/L had no significant difference.Compared with the control group,MD28 inhibited apolipoprotein(a) expression in a dose and time dependent manner,the best inhibitory effect was 1.6 g/L MD28 on 48 h.FXR and hsa-miR-23b-3p expression levels were up-regulated by MD28 while HNF4α was down-regulated in HepG2 cells,and silencing FXR reversed above-mentioned effect of MD28,silencing hsa-miR-23b-3p could also reverse the down-regulation effect of MD28 on HNF4α,but had no effect on FXR expression.Conclusion MD28 inhibits the expression of Apo(a) in HepG2 cells through the FXR/miR-23b-3p/HNF4α pathway,and is expected to be a candidate for the clinical reduction of lipoprotein(a).关键词
载脂蛋白(a)/法尼酯X受体/肝细胞核因子4α/苦瓜蛋白/miR-23b-3pKey words
Apolipoprotein (a)/Farnesyl ester X receptor/Hepatocyte nuclear factor 4α/Momordicin 28/MiR-23b-3p分类
医药卫生引用本文复制引用
马小峰,刘益洲,童海,姜淼,王佐..苦瓜蛋白通过FXR/miR-23b-3p/HNF4α途径抑制载脂蛋白(a)表达[J].中国动脉硬化杂志,2018,26(1):35-40,6.基金项目
湖南省教育厅项目(15C1201) （15C1201）
湖南省卫生计生委课题(B2016139) （B2016139）
