局解手术学杂志2017，Vol.26Issue(11)：790-795,6.DOI:10.11659/jjssx.05E017051
百草枯通过调节Wnt/β-catenin信号通路促进肺泡上皮细胞上皮间质转化
Paraquat promotes epithelial-mesenchymal transition of alveolar epithelial cells through regulating Wnt/β-catenin signaling pathway
赵春燕 1刘畅 1朱忠立 1王晓芬 1祝国芸 1李福祥1
作者信息
- 1. 成都军区总医院重症医学科,四川成都610000
- 折叠
摘要
Abstract
Objective To investigate whether paraquat (PQ) induced rat alveolar type Ⅱ cells (RLE-6TN) epithelial-mesenchymal transition(EMT) and explore the underlying molecular mechanism.Methods RLE-6TN cells were treated by 20 μmol/L PQ for 24 hours,the morphology was observed by invert microscope.RT-PCR and Western blot were performed to detect the expression level of β-catenin,EMT related markers E-cadherin and vimentin.Then we performed the Transwell invasion assays to detect the ability of cell invasion.Results The results demonstrated that PQ was able to induce the transition of RLE-6TN cells from epithelial morphology to fibroblast-like morphology,associated with the acquisition of migratory properties.Furthermore,knockdown of β-catenin by using specific siRNA could reverse PQ triggered EMT process and attenuated cell migration ability.Conclusion PQ promotes RLE-6TN epithelial-mesenchymal transition by upregulating the expression of Wnt/β-catenin.关键词
百草枯/肺纤维化/β-连环蛋白/上皮间质转化Key words
paraquat/pulmonary fibrosis/Wnt/β-catenin/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
赵春燕,刘畅,朱忠立,王晓芬,祝国芸,李福祥..百草枯通过调节Wnt/β-catenin信号通路促进肺泡上皮细胞上皮间质转化[J].局解手术学杂志,2017,26(11):790-795,6.
