局解手术学杂志2017，Vol.26Issue(11)：796-799,4.DOI:10.11659/jjssx.09E017007
Klotho通过抑制自噬逆转乳腺癌紫杉醇耐药的作用机制研究
Study on mechanism of Klotho reversing resistance of breast cancer to paclitaxel by inhibiting autophagy
摘要
Abstract
Objective To investigate the mechanism of klotho reversing the resistance of breast cancer to paclitaxel in MCF-7/PTX cells.Methods The Klotho expression in MCF-7 and MCF-7/PTX cells was detected by Western blot.The effects of Klotho on paclitaxel resistance in MCF-7/PTX cells was measured by MTT assay.The effects of Klotho and 3-methyladenine (3-MA) on proliferation and expression of Beclin1 in MCF-7/PTX cells were detected by MTT and Western blot assay,respectively.Results The expression of Klotho in MCF-7/PTX cells was decreased compared with MCF-7 cells.Klotho could sensitize MCF-7/PTX cells to paclitaxel.The expression of Beclin1 in MCF-7/PTX cells was higher than that in MCF-7 cells.Klotho and 3-MA could decrease the expression of Beclin1 in MCF-7/PTX cells,and the effects of Klotho on paclitaxel resistance in MCF-7/PTX cells was similar to that of 3-MA.Conclusion Paclitaxel resistance in breast cancer cells is related to expression of the Klotho which can reverse the resistance of breast cancer to paclitaxel by inhibiting autophagy.关键词
Klotho/自噬/乳腺癌/紫杉醇/耐药Key words
klotho/autophagy/breast cancer/paclitaxel/drug resistance分类
医药卫生引用本文复制引用
龙方懿,王婷,贾萍,王华飞,卿轶,熊婷婷,何梦婕,孙瑾,陈芳,孙倩茹..Klotho通过抑制自噬逆转乳腺癌紫杉醇耐药的作用机制研究[J].局解手术学杂志,2017,26(11):796-799,4.基金项目
四川省科技厅面上项目(2017JY0303) （2017JY0303）
四川省卫生计生委普及应用项目(17PJ562) （17PJ562）
