中国临床医学2017，Vol.24Issue(5)：681-684,4.DOI:10.12025/j.issn.1008-6358.2017.20170165
老年Wistar大鼠脂联素与脂蛋白脂肪酶的相关性
Correlation between adiponectin and lipoprotein lipase in aged Wistar rats
摘要
Abstract
Objective:To explore the relationship between adiponectin and dyslipidemia,lipoprotein lipase (LPL) in aged Wistar rats.Methods.Wistar rats were divided into the aged group (20 months old),the middle-aged group (7 months old) and the young group (3 months old) according to the age.Serum fasting blood glucose (FBG),blood lipid,and adiponectin were measured in each group.The expression levels of adiponectin mRNA and LPL mRNA in the subcutaneous adipose tissue and LPL mRNA in the liver were detected by real-time PCR.Results:With the increase of age,the expression levels of serum adiponectin and fat adiponectin mRNA,LPL mRNA and liver LPL mRNA gradually decreased;the above indexes in the aged group were significantly lower than those in the young group (P＜0.05).The levels of total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C),very low density lipoprotein cholesterol (VLDL-C),and FBG of the aged group were higher than those of the young group (P＜0.05),and the level of high density lipoprotein cholesterol (HDL-C) of the aged group was lower than that of the young group (P＜0.05).Pearson correlation analysis showed that serum adiponectin was positively correlated with fat LPL mRNA,liver LPL mRNA,and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,VLDL-C,and TG (P＜0.05).Fat adiponectin mRNA was positively correlated with fat LPL mRNA,liver LPL mRNA,serum adiponectin,and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,and VLDL-C (P＜0.05).Fat LPL mRNA was positively correlated with fat adiponectin mRNA,liver LPL mRNA,serum adiponectin and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,VLDL-C,and TG (P＜0.05).Multiple stepwise regression analysis showed that there was an independent negative correlation between serum adiponectin and VLDL-C (P＜ 0.05);there was an independent negative correlation between fat adiponectin mRNA,TC and age (P＜0.05);there was an independent negative correlation between fat LPL mRNA and age (P＜0.05).Conclusions:The level of adiponectin in subcutaneous fat and serum decreased in aged rats,and the decrease of adiponectin may be related to dyslipidemia in aged rats.It may affect the blood lipid metabolism by regulating the expression of LPL mRNA.关键词
老年/脂联素/血脂代谢/脂蛋白脂肪酶Key words
old age/adiponectin/blood lipid metabolism/lipoprotein lipase分类
医药卫生引用本文复制引用
陈灶萍,刘军,查兵兵,丁和远,吴跃跃..老年Wistar大鼠脂联素与脂蛋白脂肪酶的相关性[J].中国临床医学,2017,24(5):681-684,4.基金项目
上海市卫生和计划生育委员会青年科研项目(20114Y110).Supported by Youth Research Project of Shanghai Municipal Health and Family Planning Commission (20114Y110). （20114Y110）
