中国免疫学杂志2018，Vol.34Issue(2)：161-166,6.DOI:10.3969/j.issn.1000-484X.2018.02.001
鸭坦布苏病毒感染对BHK-21细胞分泌胞外体的影响
Effect of duck Tembusu virus infection on exosomes of BHK-21 cells
摘要
Abstract
Objective:To explore the effect of duck Tembusu virus infection on secretion of exosomes in BHK-21 cells and the pathogenesis of the Tembusu virus.Methods:The exosomes were collected and purified from the culture supernatant of BHK-21 cells infected with duck Tembusu virus AH-F10 strain and the control BHK-21 cells by PEG precipitation method respectively.The purified exosomes were identified by electron microscopy,Western blot assay and mass spectrometry.Results: The classical exosome particle morphology was observed with hyperchromic cup-shaped vesicles and average particle size of 30-160 nm in diameter under transmission electron microscopy.The mean size of the exosome from the infected cells were bigger than the mean size of the exosome from the unin-fected.Western blot assay demonstrated that CD9 and CD63 were detected in purified exosomes as exosome marker molecula.A total of 106 proteins were identified by mass spectrometry assay,84 proteins of infected BHK-21 cells exosome,49 proteins of the uninfected, and the infected and the uninfected BHK-21 share 27 common proteins on exosomes.Conclusion:Duck Tembusu virus infection affect the exosome secretion of cells in connection to the particle size and protein molecular composition.This experiment can lay the foundation for further research of Tembusu virus infection and pathogenesis.关键词
鸭坦布苏病毒/BHK-21细胞/胞外体/质谱分析Key words
Duck Tembusu virus/BHK-21 cell/Exosome/Mass spectrometry分类
农业科技引用本文复制引用
周晓雅,周祺,殷冬冬,唐井玉,邢雪,董靖,刘红梅,王桂军..鸭坦布苏病毒感染对BHK-21细胞分泌胞外体的影响[J].中国免疫学杂志,2018,34(2):161-166,6.基金项目
本文为国家重点研发计划科研专项课题(2016YFD0500805)和兽医病理生物学与疫病防控安徽省重点实验室专项资助. （2016YFD0500805）
