中国蔬菜Issue(6)：17-24,8.
甜瓜白粉病抗病基因的定位及候选基因分析
Melon Powdery Mildew Resistance Gene Location and Candidate Gene Analysis
摘要
Abstract
BC1P2 and F2generations were constructed by crossing a melon inbred line ‘MR-1’ highly resistant to powdery mildew (Podosphaera xanthii) as female parent,and a melon inbred line ‘Top Mark’highly susceptible to powdery mildew as male parent.The race of P xanthii was identified as race 1 according to the infecting action of 13 international differential hosts for melon powdery mildew,and the resistance to powdery mildew was controlled by a single dominant gene based on the population genetic analysis of BC1P2 and F2.Through evaluating the resistance in 266 individuals of F2,the genotype analysis using cleaved amplified polymorphic sequences (CAPS),and quantitative trait loci (QTL) analysis using composite interval mapping method (CIM) for locating the locus resistant to powdery mildew,a genetic linkage map of melon contained 203 CAPS was constructed.One QTL named PXR was detected on chromosome 12 between the 2 CAPS markers,M12-GH and M12-TE,which were tightly linked to the gene resistant to powdery mildew with the genetic distances of 0.63 cM and 0.42 cM,respectively.Sixty putative genes were located in the region with the length of 303 kb between the 2 flanking markers.Ten of them with non-synonymous single nucleotide polymorphism (nsSNP) were further identified as candidate genes resistant to powdery mildew in melon.The results suggested that 4 genes (MELO3C002434,MELO3C002437,MELO3C002441,and MELO3C002457) showed bigger differences in expression of disease resistance and susceptible might be the candidate genes resistant to powdery mildew in melon.关键词
甜瓜/白粉病/CAPS/抗病基因Key words
Melon/Powdery mildew/CAPS/Disease resistance gene引用本文复制引用
李冰,赵玉龙,朱强龙,张志鹏,樊超,栾非时,高鹏..甜瓜白粉病抗病基因的定位及候选基因分析[J].中国蔬菜,2017,(6):17-24,8.基金项目
国家自然科学基金项目(31301791,31672177),东北农业大学“学术骨干”计划项目(16XG06),东北农业大学“青年才俊”计划项目(14QC09),国家西甜瓜产业技术体系-分子育种岗位项目(CARS-26-02) （31301791,31672177）
