中国实验动物学报2018，Vol.26Issue(1)：8-12,5.DOI:10.3969/j.issn.1005-4847.2018.01.002
鱼腥草素钠对慢性阻塞性肺疾病模型大鼠肺组织PI3K、AKT1及mTOR mRNA表达的影响
Effect of sodium houttuyfonate on the expression of PI3K, AKT1 and mTOR mRNA in the lung of rat models of chronic obstructive pulmonary disease
摘要
Abstract
Objective To investigate the effect of sodium houttuyfonate on the expression of PI3K and AKT1 and mTOR mRNA in the lung of rats with chronic obstructive pulmonary disease(COPD),and reveal the possible mechanism of the COPD treated with sodium houttuyfonate. Methods Twenty-four male Wistar rats were randomly divided into normal control group,model control group,dexamethasone group and sodium houttuyfonate group(n=6 for each). The rat models of COPD were established by intratracheal instillation of lipopolysaccharide and smudging. The expressions of PI3K and AKT1 and mTOR mRNA were determined by real-time PCR. The morphological changes of the lung tissue was examined by histopathology. Results Compared with the normal control group,the expressions of PI3K and AKT1 were significantly in-creased and mTOR mRNA was significantly decreased in the model group(P<0.01,P<0.05). Compared with the mod-el group,the expressions of PI3K and AKT1 were significantly decreased and mTOR mRNA was significantly increased in the sodium houttuyfonate group and dexamethasone group(P<0.01,P<0.05). Compared with the dexamethasone group, the expression of mTOR mRNA was significantly increased in the sodium houttuyfonate group(P<0.05). The pathological observation indicated that there were local pulmonary consolidation and a extensive neutrophil infiltration in the alveolar cav-ity. Prominent pulmonary interstitial fibrous hyperplasia was observed in the model group. The pathological manifestations were much ameliorated than those of the model group,and only mild interstitial pneumonia and a slight fibrous hyperplasia were seen in the sodium houttuyfonate and the dexamethasone groups. Conclusions Sodium houttuyfonate reduces the in-jury of lung tissue and has protective effect on COPD rats. The mechanism is probably related to the down-regulatation of expression of PI3K and AKT1 mRNA and up-regulatation of expression of mTOR mRNA in COPD rats.关键词
鱼腥草素钠/慢性阻塞性肺疾病/磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶/雷帕霉素靶蛋白/大鼠Key words
sodium houttuyfonate/chronic obstructive pulmonary disease/PI3K/AKT1/mTOR/rats分类
生物科学引用本文复制引用
吴中华,闫玲玲,杨爱东,张海英,符胜光,杨云翔..鱼腥草素钠对慢性阻塞性肺疾病模型大鼠肺组织PI3K、AKT1及mTOR mRNA表达的影响[J].中国实验动物学报,2018,26(1):8-12,5.基金项目
国家自然科学基金面上项目(No.81673855) （No.81673855）
上海中医药大学学科能力提升项目.Funded by National Natural Science Foundation of China(No.81673855) （No.81673855）
Subject Ability Promotion Project of Shanghai University of Traditional Chinese Medicine. （）
