中国实验动物学报2018，Vol.26Issue(1)：13-19,7.DOI:10.3969/j.issn.1005-4847.2018.01.003
抑制外周神经元PAR2-PKA/PKCε通路对痛转化模型大鼠痛阈的影响
Effect of inhibition of PAR2?PKA/PKCε signaling pathway in periphery neurons on the transition from acute to chronic pain
摘要
Abstract
Objective To detect the role of PAR2-PKA/PKCε signaling pathway in periphery neurons in the tran-sition from acute to chronic pain,and investigate the possible approach to prevent both acute and chronic pain simultane-ously. Methods SD rats were randomly divided into control group,sham model group,model group,iPAR2-1 group and iPAR2-2 group. The hyperalgesia priming model was established by injection of carrageenan and PGE2 into the left hind-paw except control and sham model group. PGE2 was administrated at 7 days after carrageenan injection. The PAR2 inhibi-tor was administrated before and after PGE2 injection separately in the iPAR2-1 group and iPAR2-2 group. The paw with-drawal thresholds(PWTs)of rats in each group was detected before and at 5 h,3 d,6 d,7 d 0.5 h,7 d 4 h,7 d 24 h after carrageenan injection. The expression level of PAR2, PKA and PKCε proteins in the dorsal root ganglion(DRG) were detected at 24 h after carrageenan injection. Results The hyperalgesia priming model was successfully generated. When PGE2 was administrated at 7 days after carrageenan injection, the hyperalgesia induced by PGE2 was significantly prolonged. The PWTs of rats in the model group were significantly lower than that of the control and sham model groups(P<0.01),though the PWTs of sham model group had no significant difference with the control on 7 d 24 h after carrageenan injection(P>0.05). The expression level of PAR2 and PKCε in the ipsilateral DRG neurons were significantly increased on 7 d 24 h after carrageenan injection,when compared with the control and sham model groups(P<0.05). PAR2 inhibi-tor prevented the prolonged hyperalgesia induced by PGE2(P<0.05)and decreased the PKCε expression in DRG neurons whenever it was given(P<0.05). However,PAR2 inhibitor did not regulate the acute inflammatory pain of PGE2 and the expression of PKA in DRG neurons(P>0.05). Conclusions Inhibition of the expression of PAR2 can prevent the tran-sition from acute to chronic pain. This effect may be related with the inhibitory effect on the activation of PAR2-PKCε sig-naling pathway in DRG neurons. However,inhibition of PAR2 can not regulate the acute pain. These may because of that the PAR2-PKA signaling pathway does not play a role in acute pain.关键词
痛转化/慢性痛/背根神经节/酶激活受体2/蛋白激酶Cε/大鼠Key words
transition from acute to chronic pain/chronic pain/dorsal root ganglion/PAR2/PKCε/rats分类
生物科学引用本文复制引用
房军帆,王思思,孙海榉,邵晓梅,梁宜,方剑乔,杜俊英..抑制外周神经元PAR2-PKA/PKCε通路对痛转化模型大鼠痛阈的影响[J].中国实验动物学报,2018,26(1):13-19,7.基金项目
浙江省科技厅公益性(实验动物平台)项目(No.2016C37135) （实验动物平台）
国家自然科学基金青年项目(No.81603692) （No.81603692）
国家自然科学基金青年项目(No.81603690) （No.81603690）
浙江省医药卫生一般研究计划(No.2016KYA154).Funded by Zhejiang Province Public Welfare(experimental animal platform)Project(No.2016C37135) （No.2016KYA154）
Youth Fund of National Natural Science Foundation Projects(No.81603692) （No.81603692）
Youth Fund of National Natural Science Foundation Projects(No.81603690) （No.81603690）
Zhejiang General Research Pro-gram on Medical and Health(No.2016KYA154). （No.2016KYA154）
