中国实验动物学报2018，Vol.26Issue(1)：52-56,5.DOI:10.3969/j.issn.1005-4847.2018.01.008
亲和吸附材料特异性清除肠源性内毒素有效性和安全性探讨
Efficacy and safety of the removal of intestinal endotoxin in the blood by affinity adsorption materials
摘要
Abstract
Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intes-tinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perfo-ration of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment,we moni-tored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was(0.49 ± 0.22),(0.034 ± 0.00)Eu/mL after hemoperfusion for 1 and 2 hours,compared with that before the beginning of perfusion(7.25 ± 1.18)Eu/mL,showing a significant difference(P <0.05). After treatment for 2 hours,the average clearance rate was 99.52%. White blood cells,red blood cells,hemoglo-bin,platelets,total protein,albumin,globulin,alanine aminotransferase,aspartate aminotransferase,urea and creatinine index were significantly decreased after perfusion(P < 0.05). The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively re-move endotoxin in the blood of experimental dogs and has good blood compatibility.关键词
内毒素/肠源性内毒素血症/血液灌流/亲和吸附材料/犬Key words
endotoxin/intestinal endotoxin/hemoperfusion/affinity adsorption material/dogs分类
生物科学引用本文复制引用
向勇平,崔辉,刘丹,于杰,周立冬,杨建明,刘爱兵..亲和吸附材料特异性清除肠源性内毒素有效性和安全性探讨[J].中国实验动物学报,2018,26(1):52-56,5.基金项目
国家高技术研究发展计划(No.2015AA033904).Funded by National High Technology Research and Development Plan(No.2015AA033904). （No.2015AA033904）
