中国实验动物学报2018，Vol.26Issue(1)：65-71,7.DOI:10.3969/j.issn.1005-4847.2018.01.010
大气细颗粒物致炎动物模型系统评价
Systematic evaluation of pulmonary inflammation induced by fine particulate matter in a mouse model
摘要
Abstract
Objective The aim of this study was to establish a PM2.5air pollution-induced mouse model of pulmo-nary inflammation and investigate its pathogenetic mechanism. Methods 150 specific pathogen-free BALB/c mice were subjected to intratracheal instillation of 2.5,5,or 10 mg/kg PM2.5suspension to construct airborne inflammation models. The blank group and saline group were taken as a control group. Mice were euthanized after 3rd,7th,21st,35th and 49th days to assess the pathological changes in lung tissues using HE staining and ELISA. Results The success rate of tracheal instillation was 96%. With the time prolongation and increasing doses of intratracheal PM2.5instillation,the histopathologi-cal scores of lung tissue increased gradually,showing alveolar macrophages with engulfed particles and lymphocyte accumu-lation in bronchiole and widened inter-alveolar space. The levels of BALF IL-6 and TNF-α of lung tissue homogenate were significantly increased in the high dose PM2.5(10 mg/kg)group, compared with the control groups. Conclusions A mouse model of PM2.5air pollution-induced lung inflammation is successfully established by intratracheal instillation of PM2.5suspension.This method is proved to be simple,safe and reliable,and is useful for further study of air pollution-in-duced and other inflammatory mechanisms.关键词
PM2.5/大气污染/气管滴注/模型/小鼠Key words
PM2.5/air pollution/intratracheal instillation/model/mice分类
生物科学引用本文复制引用
侯天芳,马元元,王广发..大气细颗粒物致炎动物模型系统评价[J].中国实验动物学报,2018,26(1):65-71,7.基金项目
国家自然科学基金面上项目(No.81370106) （No.81370106）
首都卫生发展专项研究重点攻关项目(No.2016-1-4071) （No.2016-1-4071）
国家重点研发计划:慢病专项课题(No.2017YFC1309500) （No.2017YFC1309500）
北京市自然科学基金重点项目(No.7161013) （No.7161013）
北京大学临床研究项目项目编号(PUCRP201303).Funded by National Natural Science Foundation of China(No.81370106) （PUCRP201303）
Capital Medical Development and Scientific Research Fund(No.2016-1-4071) （No.2016-1-4071）
The State Key Development Program(No.2017YFC1309500) （No.2017YFC1309500）
Beijing Municipal Natural Science Foundation(No.7161013) （No.7161013）
Peking Uni-versity Clinical Research Project(No.PUCRP201303). （No.PUCRP201303）
