中国实验动物学报2018，Vol.26Issue(1)：79-85,7.DOI:10.3969/j.issn.1005-4847.2018.01.012
食蟹猴肌肉萎缩模型的建立
Establishment of a cynomolgus monkey model of gastrocnemius muscle atrophy
摘要
Abstract
Objective To establish a monkey model of muscle atrophy by fixing the right lower limb and set up e-valuation criteria for that model. Methods Twelve healthy,6-7 year old male cynomolgus monkeys(body weight 6-7 kg)were used in this study. All animals were normal and had no malformed lower limb bones. Right lower limbs of the an-imals were fixed with fiberglass bandage(from knee joint to anklebone)for 15 weeks. Body weight,crus perimeter and crus volume were recorded every two weeks. MRI examinations of the gastrocnemius muscle were conducted at weeks 11,13 and 15. Muscle biopsy was taken for pathological examination at week 15. Results There were no obvious changes of body weight during the experiment. At 15 weeks after modeling,the right crus perimeter and crus volume of the experimental an-imals were significantly decreased(P <0.05),and some biochemical indexes such as serum creatinine,gloucose and al-kaline phosphatase were significantly decreased(P <0.05), while cholesterol was significantly increased(P <0.05).MRI showed atrophy of the right gastrocnemius muscle. Muscle biopsy also showed muscle atrophy and muscle fibers be-came thinner in the right gastrocnemius,and the cross-section area of the muscle fibers was significantly decreased. Con?clusions It is easy to operate and establish a muscle atrophy model in Cynomolgus monkeys by fixing the right lower limb. This model is suitable for objective and easy evaluation of muscle atrophy by measurement of crus perimeter and crus volume of the limb,blood biochemical parameters,MRI examination,and histopathological examination in combination.关键词
食蟹猴/肌肉萎缩/MRI/组织病理学/血液生化Key words
cynomolgus monkey/muscle atrophy/MRI/histopathology/blood biochemisty分类
生物科学引用本文复制引用
刘雪萍,李振明,邓信宁,何乃知,杨继红..食蟹猴肌肉萎缩模型的建立[J].中国实验动物学报,2018,26(1):79-85,7.基金项目
"重大新药创制"科技重大专项课题子课题(No.2011ZX09307-303-02) （No.2011ZX09307-303-02）
广西中青年教师基础能力提升项目(No.KY2016YB243).Funde by National Science and Technology Major Projects for"Major New Drugs Innovation and Development"(No.2011ZX09307-303-02) （No.KY2016YB243）
Guan-gxi Youth Teachers&apos （）
Basic Ability Promotion Project(No.KY2016YB243). （No.KY2016YB243）
