中国实验动物学报2018，Vol.26Issue(1)：101-106,6.DOI:10.3969/j.issn.1005-4847.2018.01.016
大鼠腰椎腹侧神经根牵拉模型的建立
Establishment of a rat model of lumbar spinal root avulsion
刘吉宏 1葛立军 2陈焱 2张勇 2陆嘉琦 2刘爱莲 2李宁 2孙怀昌 2王建飞1
作者信息
- 1. 扬州大学兽医学院,扬州 225009
- 2. 葛兰素史克(上海)医药研发有限公司,整合生物科技平台,上海 201203
- 折叠
摘要
Abstract
Objective To establish a rat model of spinal root avulsion and to validate the model by brain-derived neurotrophic factor(BDNF)treatment. Methods To evaluate the motor neuron loss,5 male SD rats were used to undergo spinal root avulsion surgery. One week later, the number of motor neurons in the ventral horn of the spinal cord was as-sessed by histopathology using immunohistochemical staining with a choline acetyl transferase(ChAT)antibody. After this pilot study,40 male SD rats at 7 weeks of age were randomly divided into 4 groups:two control groups,BDNF preventive and treatment groups. Results All rats recovered well post-surgery and no obvious abnormality was observed. Compared with the contralateral side,the number of motor neurons in the ipsilateral avulsed side was significantly decreased at one week after surgery(20.06%,P<0.05). Compared with the control group,there was a significant increase in ChAT posi-tive neurons in the BDNF preventive group(17.85% vs. 93.06%,P<0.0001)or BDNF treatment group(1week after surgery)(26.94% vs. 86.87%, P<0.0001), indicating that the motor neurons were effectively protected by BDNF. Conclusions A rat model of spinal root avulsion is successfully established,which can be valuable for studies of amyotro-phic lateral sclerosis and drug discovery efforts.关键词
神经根牵拉/脑源性神经因子/肌萎缩侧索硬化症/大鼠Key words
spinal root avulsion/brain-derived neurotrophic factor/BDNF/amyotrophic lateral sclerosis/rats分类
生物科学引用本文复制引用
刘吉宏,葛立军,陈焱,张勇,陆嘉琦,刘爱莲,李宁,孙怀昌,王建飞..大鼠腰椎腹侧神经根牵拉模型的建立[J].中国实验动物学报,2018,26(1):101-106,6.
