中国实验动物学报2018，Vol.26Issue(1)：107-113,7.DOI:10.3969/j.issn.1005-4847.2018.01.017
金思维对东莨菪碱致记忆障碍小鼠脑胆碱能系统的影响
Effect of herbal formula GAPT on cholinergic system in mice with scopolamine?induced memory impairment
摘要
Abstract
Objective To investigate the effect of a Chinese medicine formula GAPT, a combination containing Chinese herbs ginseng,Acorus tatarinowii, Polygala and tuber curcuma, on the behavior and cholinergic system in mice with scopolamine-induced memory impairment, and to explore the neuroprotective mechanism of GAPT. Methods ICR mice were randomly divided into normal control group(solution of 0.5% carboxy methyl cellulose, CMC), model group (0.5% CMC),positive control group(donepezil, 0.92 mg/(kg·d)), GAPT high, medium and low dose groups(20 mg/(kg·d),10 mg/(kg·d),5 mg/(kg·d)),18 mice in each group, were given intragastric gavage once a day for 30 days. After the last administration,the control group and model group received intraperitoneal injection of normal saline,and the other groups intraperitoneal injection of scopolamine(3 mg/(kg·d)), dissolved in 0.9% normal saline. Morris water maze test was performed to assess the learning and memmory ability. Then the mice were killed and the Ach content and AchE and ChAT activity in the cortex and hippocampus were detected. Results GAPT increased the swimming dis-tance,swimming time and the residence time in target quadrant of the model mice,increased the content of Ach and the ac-tivity of ChAT,and decreased the activity of AchE in the brain of the model group. Conclusions GAPT can improve the learning and memory ability of mice induced by scopolamine,and its mechanism may be related to cholinergic system.关键词
金思维/阿尔茨海默病/乙酰胆碱/乙酰胆碱酯酶/胆碱乙酰转移酶/小鼠Key words
GAPT/a Chinese medicine formula/Alzheimer&apos/s disease/acetylcholine/acetylcholinesterase/cho-lineacetyltransferase/mice分类
生物科学引用本文复制引用
董云芳,冯慧利,陈芳,盛宁,任映,杨金铎,时晶,田金洲,王蓬文..金思维对东莨菪碱致记忆障碍小鼠脑胆碱能系统的影响[J].中国实验动物学报,2018,26(1):107-113,7.基金项目
北京中医药大学在读研究生项目(No.2016-JYB-XS142) （No.2016-JYB-XS142）
北京市教育委员会科学研究与研究生培养共建项目(No.2016,2017) （No.2016,2017）
国家自然科学基金面上资助项目(No.81573927).Funded by Graduate Program of Beijing University of Chinese Medicine(No.2016-JYB-XS142) （No.81573927）
The Beijing Municipal Commission of Education Sci-entific research and Graduate Training Co-Construction Project(2016,2017) （2016,2017）
National Natural Science Foundation of China(No.81573927). （No.81573927）
