中国兽医科学2018，Vol.48Issue(2)：142-147,6.DOI:10.16656/j.issn.1673-4696.2018.0022
动物衣原体RPA检测方法的建立
Detection of Chlamydia in livestock by recombine polymerase amplification
摘要
Abstract
The aim of this study was to establish a rapid detection method for Chilamydia in abortive animals to facilitate timely treatment.A recombines polymeraseamplification(RPA) method was developed using primers and a probe specific for the conserved gene of envB.The RPA reaction was successfully performed at 37-39℃ for 20 min.With recombinant plasmid DNA carrying envBgene as template,the test results showed that the sensitivity of RPA was 101copies of plasmid DNA.Positive rate for Chlamydia detection of i0 clinical samples by RPA was consistent with that by conventional PCR.The established RPA method is simple,rapid,and reliable,which can be utilized as a novel tool for experimental research and detection of Chlamydial infection in clinical samples.关键词
重组酶聚合酶扩增/衣原体/快速检测Key words
recombines polymerase amplification/Chlamydia/rapid detection分类
农业科技引用本文复制引用
费媛媛,李兆才,娄忠子,Muhammed MUSTAFA,张志君,赵宏涛,祁世荣,李长云,周继章..动物衣原体RPA检测方法的建立[J].中国兽医科学,2018,48(2):142-147,6.基金项目
“十三五”国家重点研发计划“牛羊重要病原体分子检测新技术研究”(2016YFD0500907) （2016YFD0500907）
