中国兽医科学2018，Vol.48Issue(2)：161-166,6.DOI:10.16656/j.issn.1673-4696.2018.0025
禽白血病病毒K亚群RT-LAMP检测方法的建立
Development of RT-LAMP method for the detection of avian leukosis virus subgroup K
摘要
Abstract
Based on the gp85 gene sequences of avian leukosis virus subgroup K from GenBank database,the specific RT-LAMP primers were designed to detect avian leukosis virus subgroup K (ALV-K).The different concentration combinations of inner and outer primers were tested and reaction temperature was optimized to establish the RT-LAMPmethod for detecting ALV-K.The results showed that this method can specifically detect ALV-K without cross-reactions with other subgroups of ALV or other poultry disease pathogens such as NDV,REV,AIV,MDV,IBV,etc..The sensitivity test indicated that the minimum detectable limit was 3.4× 104copies of plasmids.关键词
禽白血病病毒/K亚群/RT-LAMP/检测方法Key words
avian leukosis virus/subgroup K/RT-LAMP/test method分类
农业科技引用本文复制引用
黄海超,陈轩,杨素,赵海燕,曹伟胜,邵建宏,赵福振,沙才华,李忠..禽白血病病毒K亚群RT-LAMP检测方法的建立[J].中国兽医科学,2018,48(2):161-166,6.基金项目
国家自然科学基金项目(31672552) （31672552）
广东省自然科学基金项目(2016A030313403) （2016A030313403）
广东省家禽产业技术体系疾病控制岗位专家项目(2016LM1114) （2016LM1114）
国家肉鸡产业技术体系项目(CARS-41-G16) （CARS-41-G16）
2015年国家质量监督检验检疫总局科技计划项目(2015IK337) （2015IK337）
珠海出入境检验检疫局科技项目(ZH2016-6) （ZH2016-6）
