中国兽医科学2018，Vol.48Issue(2)：189-195,7.DOI:10.16656/j.issn.1673-4696.2018.0029
旋毛虫cystatin-like基因重组蛋白诊断特性的鉴定
Diagnostic identification of cystatin-like gene recombinant protein in Trichinella spiralis
摘要
Abstract
To identify the diagnostic characteristics of cystatin-like gene protein of the intestinal stage larvae of Trichinella spiralis,ELISA was used using the cystatin-like gene recombinant protein as envelope antigen and the excretory-secretory (ES) antigen of early intestinal 6 hours muscle larvae (6hML) was made as positive control to detect the level of anti-Trichinella antibody IgG with different infection doses and days post infection (dpi).The antibody IgG rules were mapped by Microsoft Excel,which indicated the sensitivity and the effects for diagnosis of cystatin-like gene recombinant protein.The results showed that,in all groups,the anti-Trichinella antibody IgG was positive steadily from the 10 dpi and 8 dpi;the curve of antibody IgG was "crest wave trough"type and maintained at a high level from ii dpi to 45 dpi;antibody IgG level was significantly higher at the early stage (from 15 dpi to 45 dpi) in low dose group.Therefore,cystatin-like gene recombinant protein has excellent diagnostic ef fects for early serodiagnosis,especially in low dose infection,and it is speculated that cystatin-like gene is likely to play an important role in host immune inhibition at a early stage of Trichinella infection.Biology functions of cystatin-like gene recombinant protein remain to be further studied.关键词
旋毛虫/cystatin-like基因/基因重组蛋白/早期诊断Key words
Trichinella spiralis/cystatin-like gene/gene recombinant protein/early diagnosis分类
农业科技引用本文复制引用
翟铖铖,陈家旭,陈韶红,艾琳,卢艳,蔡玉春,俞英昉,田利光,吴秀萍..旋毛虫cystatin-like基因重组蛋白诊断特性的鉴定[J].中国兽医科学,2018,48(2):189-195,7.基金项目
国家重点研发计划项目(2017YFD0501303) （2017YFD0501303）
