中国兽医科学2018，Vol.48Issue(2)：196-203,8.DOI:10.16656/j.issn.1673-4696.2018.0030
猪繁殖与呼吸综合征病毒河南株HNxy的分离鉴定及遗传进化分析
Isolation,identification and genetic variation analysis of Henan strain HNxy of porcine reproductive and respiratory syndrome virus
摘要
Abstract
To understand the evolution of PRRSV in Henan,a PRRSV strain was isolated in Marc-145 cells from the lung of suspected PRRSV-positive pig in Xinyang of Henan,and named as HNxy.HNxy strain could cause typically cytopathic effects.The results ofphylogenetic tree and homology analysis showed that HNxy belonged to the same cluster with high pathogenic PRRSV (HP-PRRSV)strains in China.The nucleotide homology of HNxy was the lowest with Lelystad virus,only up to 60.0％,and 89.2％ with American classic strain VR-2332.The nucleotide homology of HNxy with Chinese HP-PRRSV strains JXwn06,JXA1 and HuN4 were 98.4％,98.3％ and 98.4％,respectively.HNxy had the highest nucleotide homology with JXA1-P80(98.7％).The nucleotide homology of HNxy with NADC30 and NADC30-like strains HENAN-HEB,JL580,CHsx1401 were 84.6％,84.7％,87.3％ and 83.6％,respectively.The results of sequence alignment showed that there were 30 discontinuous amino acid(aa) deletions in the high variable region of NSP2 and the epitopes of GP5 had some aa mutations.These results showed that HNxy belonged to a vaccine strain of highly pathogenic North American type PRRSV and had some mutations in the epitope region.关键词
猪繁殖与呼吸综合征病毒/分离鉴定/进化分析Key words
porcine reproductive and respiratory syndrome virus/isolation and identification/genetic variation analysis分类
农业科技引用本文复制引用
董建国,张广强,赵瑜,胡静,郭晓秋,王瑞,焦凤超,赵攀登..猪繁殖与呼吸综合征病毒河南株HNxy的分离鉴定及遗传进化分析[J].中国兽医科学,2018,48(2):196-203,8.基金项目
阿南省科技攻关项目(172102110198) （172102110198）
2015年学校青年教师基金项目(201501002) （201501002）
预防兽医学教学团队项目(JXTD201701) （JXTD201701）
