中国兽医科学2018，Vol.48Issue(2)：204-210,7.DOI:10.16656/j.issn.1673-4696.2018.0031
MicroRNA-181a对日本血吸虫童虫抗原刺激巨噬细胞的免疫应答起负调节作用
MicroRNA-181a plays a negative immune regulation role in macrophages stimulated with soluble schistosomula antigens of Schistosoma japonicum
摘要
Abstract
Macrophages RAW264.7 were stimulated and/or transformed with soluble schistosomula antigens(SSA) of Schistosoma japonicum and/or miR-181a mimics.The expression level of miR-181a,TNF-α and other cytokines,as well as M1 and M2 cell marker molecules were analyzed by real-time quantitative PCR or flow cytometry.The results showed that the expression of miR-181a,IL-1β,IL-4,TNF-α,IL-10 and IL-6 was up-regulated when the cells were stimulated with SSA.While IL-6 and IL-1β,TNF-α and INOS were down-regulated,IL-4 and arg-1 were up-regulated after RAW264.7 macrophages were transfected with miR-181a mimics alone.And the expression of IL-1β,IL-4,TNF-α,IL-10 and IL-6 was all down-regulated when the macrophages were first stimulated with SSA then transformed with miR-181a mimics.The results of flow cytometry showed that miR-181a mimics could down-regulate the expression of CD16/32,and up-regulate the expression of CD206.This study suggests that miR-181a may play a negative immune regulation role in macrophages stimulated with SSA,and promote the transformation of M1 to M2 macrophages.关键词
日本血吸虫/miR-181a/SSA/巨噬细胞Key words
Schistosoma japonicum/miR-181a/SSA/macrophage分类
农业科技引用本文复制引用
唐仪晓,沈元曦,洪炀,翟颀,张祖航,韩宏晓,傅志强,林矫矫..MicroRNA-181a对日本血吸虫童虫抗原刺激巨噬细胞的免疫应答起负调节作用[J].中国兽医科学,2018,48(2):204-210,7.基金项目
国家自然科学基金资助项目(81401692) （81401692）
国家重点研发计划项目(2017YFD0501306) （2017YFD0501306）
