中国兽医科学2018，Vol.48Issue(2)：222-227,6.DOI:10.16656/j.issn.1673-4696.2018.0034
铅暴露对鲫鱼抗氧化及免疫能力的影响
Effects of lead exposure on antioxidation and immunity of Carassius aurous
摘要
Abstract
In order to study the effects of different mass concentration of lead exposure on the antioxidation and immunity of Carassius aurous,180 C.aurous were randomly divided into four groups:control group Ⅰ,lead exposure groupⅡ(0.05 mg/L),lead exposure group Ⅲ(0.5 mg/L) and lead exposure group Ⅳ(1 mg/L).The activities of superoxide dismutase(SOD) and catalase(CAT),glutathione(GSH) mass concentration,total antioxidant capacity(T-AOC) in the liver,serum lysozyme(LZM) activity and immunoglobulin M(IgM) mass concentration were evaluated after 30 d and 60 d exposure.The results showed that after 30 days of lead exposure,compared with the control group,the levels of T-AOC in groupsⅡ and Ⅳwere significantly increased(P＜0.05),and that in group Ⅲ was slightly decreased(P＞0.05);the levels of SOD in groupsⅡ,Ⅲ and Ⅳ were significantly increased(P＜0.05);the levels of GSH in groupsⅡ and Ⅲ were significantly decreased(P＜0.05),and it was significantly increased in group Ⅳ (P＜0.05).The levels of CAT,LZM and IgM in groupsⅡ,Ⅲ and Ⅳ were significantly decreased(P＜0.05).After 60 days of lead exposure,compared with the control group,the levels of T-AOC and GSH in groups Ⅱ,Ⅲ and Ⅳ were significantly increased(P＜0.05),the levels of SOD in groups Ⅱand Ⅳ were significantly increased,the levels of SOD in group Ⅲ were increased,but the difference was not significant(P＞0.05);the levels of CAT were significantly decreased in groups Ⅱ,Ⅲ and Ⅳ (P＜0.05);the levels of LZM and IgM in groups Ⅱ and Ⅳ were significantly increased(P＜0.05),the levels of LZM in group Ⅲ were significantly decreased(P＜0.05),and the level of IgM was not significantly increased(P＞0.0S).It can be seen that lead exposure can induce oxidative stress in crucial carp and affect the immune system,and there are differences in antioxidant and immune indexes in different mass concentration groups.Colossians reduce body damage by lead exposure via regulating their anti-oxidation and immune defense system.关键词
鲫鱼/铅暴露/SOD/GSH/CAT/T-AOC/LZM/IgMKey words
Carassius aurous/lead exposure/SOD/GSH/CAT/T-AOC/LZM/IgM分类
农业科技引用本文复制引用
郭曦尧,尹玉伟,茆安婷,代静,程义,张蕾,张培军,李月红..铅暴露对鲫鱼抗氧化及免疫能力的影响[J].中国兽医科学,2018,48(2):222-227,6.基金项目
国家自然科学基金项目(30972191) （30972191）
国家引进国际先进农业科学技术计划(948)项目(2014Z34) （948）
吉林省教育厅“十二五”科学技术研究重点项目(201443) （201443）
