中国兽医科学2018，Vol.48Issue(2)：228-233,6.DOI:10.16656/j.issn.1673-4696.2018.0035
屎肠球菌对鲤鱼非特异性免疫酶活性和抗病力的影响
Effects of Enterococcus faecium on non-specific immuno-enzyme activity and resistance in carp
摘要
Abstract
To investigate the effects of the feed of Enterococcusfaecium on non-specific immunity and disease resistance in carp,three different concentration of E.faecium(1 × 107CFU/g,1 × 10SCFU/g,1× 109CFU/g) was divided into four groups(n=20),and the initial body weight of carp was 28 g±0.3 g.The results showed that the activity of SOD increased significantly,but the activities of LZM,POD,AKP and mass contentration of IgMwere also increased but non significantly(P＞0.05).TheAKPand POD activities(1 × 108 CFU/g) were the highest 28 day post-1st-immunization(dpi).The LZM and SOD activities (1 × 108CFU/g) were the highest 35 dpi,and those of 1× 107CFU/g and 1 × 109 CFU/g were lower than that of 1 × 108CFU/g,and all the experimental group were higher than the control group.The IgM content was significantly higher than that in serum PBS(P＜0.05),and the highest level of serum IgM was found 14 dpi.On the 42nd day after feeding,fishes were challenged with Aeromonas veronii strain TH0426,and there were significant differences in the cumulative mortality after 14 days.Compared with the control group,the immune protection rate of E.faecium with 1× 108 CFU/g was 58％,followed by 1 × 107CFU/g(42％)and 1 × 109 CFU/g(38％).Thus,adding 1× 108 CFU/g and 1 × 109CFU/g of E.faecium to the basal diet could improve the non-specific immunity of carp and higher immune protection effects.关键词
屎肠球菌/鲤鱼/酶活力/免疫效果Key words
Enterococcus faecium/carp/enzyme activity/immune effect分类
农业科技引用本文复制引用
鞠安琪,谷巍,张海朋,曲磊,张冬星,陈龙,康元环,单晓枫,钱爱东..屎肠球菌对鲤鱼非特异性免疫酶活性和抗病力的影响[J].中国兽医科学,2018,48(2):228-233,6.基金项目
国家自然科学基金项目(31201927) （31201927）
吉林省重点科技攻关项目(20150204065NY) （20150204065NY）
