中国兽医科学2018，Vol.48Issue(2)：240-246,7.DOI:10.16656/j.issn.1673-4696.2018.0037
卵泡期和黄体期牦牛输卵管中Bcl-2和Bax的表达变化
Difference of Bcl-2 and Bax expression in the yak oviduct during follicular and luteal phases
摘要
Abstract
The methods of Streptavidin-Perosidase(SP) immunohistochemistry and real-time fluorescent quantitative PCR were used to study the expression of Bcl-2 and Bax in the yak oviduct in a follicular and luteal phase.The results showed that the Bcl-2 and Bax proteins were expressed in the cytoplasm of the oviduct epithelium,and their immunoreactivity was mainly detected in the free and basal surface of the epithelial cells.In the luteal phase,Bax immunoreactivity was significant higher than that in the follicular phase,but Bcl-2 immunoreactivity in the luteal phase were decreased than that in the follicular phase with no significance.In follicular and luteal phases,Bcl-2 and Bax mR NA were expressed in the yak oviduct,and Bcl-2 expression had no significant difference in follicular and luteal phases,but at the luteal phases,Bax was significantly higher than that in the follicular phase.The results suggested that Bcl-2 and Bax are involved in the regulation of cyclic cell apoptosis of the yak oviduct epithelium,and for this apoptosis pathway,it takes effects mainly through the regulation of Bax expression.关键词
牦牛/输卵管/Bcl-2/BaxKey words
yak/oviduct/Bcl-2/Bax分类
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阿依木古丽·阿不都热依木,任瑞,乔自林,李卓,李赛洋,邓阿成,刘喆,蔡勇..卵泡期和黄体期牦牛输卵管中Bcl-2和Bax的表达变化[J].中国兽医科学,2018,48(2):240-246,7.基金项目
国家自然科学基金项目(31260589) （31260589）
中央高校基本业务费专项资金“一优三特”学科建设专项经费项目(31920170174,31920170170) （31920170174,31920170170）
