中国兽医科学2018，Vol.48Issue(2)：262-268,7.DOI:10.16656/j.issn.1673-4696.2018.0040
玉米赤霉烯酮对大鼠睾丸支持细胞Fas/FasL凋亡信号通路的影响
Effects of zearalenone on apoptosis via the Fas/FasL pathway in sertoli cells
摘要
Abstract
In order to investigate the apoptosis of sertoli cell(SC)induced by zearalenon(ZEA),the primary SC was used as the experiment material,and exposed to different concentration of ZEA(0,5,10,20μmol/L)for 24 h.The CCK-8 method was used to estimate cells viability;the flow cytometry was used to detect an apoptotic rate.The level of gene transcription of Fas and FasL was detected by qRT-PCR and the expression of apoptosis-related proteins was detected by Western-blot.The results showed that,compared with the control group,the viability of the SC in the exposed group was inhibited (P＜0.05 or P＜0.01) with the increase of ZEA concentration.The flow cytometry showed that the apoptotic rate was increased significantly(P＜0.01)in ZEA group.The qRT-PCR results showed that the level of gene transcription of Fas and FasL was increased significantly with 10,20μmol/L of ZEA(P＜0.05 or P＜0.01).Westernblot analysis showed that the expression of Fas,FasL,FADD,Cleaved-caspase8 and C]eaved-caspase3 was increased in cells treaed with 5μmol/L or more ZEA(P＜0.05 or P＜0.01).All these results clarified that ZEA would induce the apoptosis of sertoli cell via the Fas/FasL pathway.关键词
玉米赤霉烯酮/睾丸支持细胞/Fas/FasL途径/大鼠Key words
zearalenone/sertoli cell/Fas/FasL pathway/rat分类
农业科技引用本文复制引用
司梦雪,卞建春,刘利,王玲玲,王涛,邹辉,顾建红,袁燕,刘学忠,刘宗平..玉米赤霉烯酮对大鼠睾丸支持细胞Fas/FasL凋亡信号通路的影响[J].中国兽医科学,2018,48(2):262-268,7.基金项目
国家重点研发计划支持项目(2016YFD0501208) （2016YFD0501208）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
