中国实用口腔科杂志2017，Vol.10Issue(12)：732-735,739,5.DOI:10.19538/j.kq.2017.12.006
槟榔碱对Hacat细胞上皮模型通透性的影响及钙离子的调节作用研究
Effect of arecoline on the permeability of Hacat cell epithelia model and the regulation of calcium ion
摘要
Abstract
Objective To study the effect of arecoline on the permeability of Hacat cell epithelia model in vitro and the adjustment of Ca2+ on it.Methods Establish the Hacat cell epithelia monolayer model in vitro by culturing Hacat cell.The models were divided into control group (group A),arecoline treatment group (group B) and "calcium+ arecoline" treatment group (group C).The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatment with arecoline and calcium.Results In group B,the Lucifer Yellow Papp of each subgroup was higher than that of group A (P ＜ 0.05) and the Lucifer Yellow Papp increased as the arecoline concentration and treatment time were increased (P ＜ 0.05).In group C,after pretreatment with calcium,the Lucifer Yellow Papp of each subgroup were lower than that of group B at corresponding concentration and time points (P ＜ 0.05);while the Papp of each subgroup was higher than that of group A (P ＜ 0.05).Besides,the Lucifer Yellow Papp increased as the arecoline concentration and treating time were increased (P ＜ 0.05).Conclusion The increase of permeability of oral epithelia model by arecoline may play an important role in the pathogenesis and development of oral submucous fibrosis.The pretreatment with calcium,to some extent,can weaken the effect of arecoline on the permeability of Hacat cell monolayer model.关键词
口腔黏膜下纤维性变/槟榔碱/钙离子/通透性/Hacat细胞Key words
oral submucous fibrosis, OSF/arecoline/calcium ion/permeability/Hacat cells分类
医药卫生引用本文复制引用
陈蓉,侯冬兰,尹晓敏,高义军..槟榔碱对Hacat细胞上皮模型通透性的影响及钙离子的调节作用研究[J].中国实用口腔科杂志,2017,10(12):732-735,739,5.基金项目
湖南省自然科学基金资助项目(14JJ2030) （14JJ2030）
