中国现代医学杂志2018，Vol.28Issue(6)：9-14,6.DOI:10.3969/j.issn.1005-8982.2018.06.002
miRNA-381对胃癌细胞增殖和侵袭的影响及机制
Role of miR-381 on cell proliferation and invasion of gastric cancer
摘要
Abstract
Objective To investigate the role of miR-381 on proliferation and invasion of gastric carcinoma cells and its potential mechanism. Methods The expression of miR-381 in normal and gastric cell lines were determined by qRT-PCR. The AGS cell line was divided into two groups including negative control group which was transfected with miR-381 vehicle, and miR-381 mimics group which was transfected with miR-381 mimics through Lipofectamine 2000. Proliferation and invasion rate were evaluated with CCK8 and Transwell assay, respectively. LRH-1 and Twist1 protein levels were measured by Western blot. Results Substantially reduction of miR-381 was found in gastric cancer cell line when compared with normal gastric cell line (P < 0.05). The number of invasive cell in miR-381 mimics group was decreased significantly compared with negative control group [(31.70 ± 4.2) vs (79.6 ± 7.2), P < 0.01]. Expression levels of LRH-1 and Twist1 significantly dropped in miR-381 mimics group in comparison with those in negative control group [(0.39 ± 0.04) vs (1.0 ± 0.0), (0.51 ± 0.05) vs (1.0 ± 0.05), P < 0.01, respectively]. Conclusions Low expression of miR-38 is identified in gastric cell line. Up-regulated expression of miR-381 contributes to inhibition of cellular proliferation and invasion activity through LRH-1and Twist signal pathway.关键词
胃癌/miRNA-381/增殖/侵袭/肝受体类似物1/扭曲相关蛋白1Key words
gastric cancer/miR-381/proliferation/invasion/LRH-1/Twist1分类
医药卫生引用本文复制引用
李丽平,叶俊,吴舜,马松林,周艳玲..miRNA-381对胃癌细胞增殖和侵袭的影响及机制[J].中国现代医学杂志,2018,28(6):9-14,6.基金项目
湖北省卫生厅资助课题(No:WJ2017M177) （No:WJ2017M177）
