中国现代医学杂志2018，Vol.28Issue(6)：38-44,7.DOI:10.3969/j.issn.1005-8982.2018.06.007
外泌体在前列腺癌发展和转移中的作用
Role of exosomes in development and metastasis of prostate cancer
摘要
Abstract
Objective To investigate the concentration of circulating exosomes in prostate cancer patients, elucidating role of exosomes in the development and metastasis of prostate cancer. Methods Circulating exosomes from human beings were isolated via centrifugation. Concentration of exosomes were determined by BCA assay. Expression of CD63 were measured by Immunohistochemistry staining. MTT assay and Western blotting were performed to identify activity of proliferation and exosomes secretion. Mouse model of lung metastasis was established to examine effect of exosomes on distant metastasis ability. Expression of CD63 and Ki-67 in pulmonary tumor tissue were determined by Immunofluorescent staining. Results The concentration of exosomes in prostate cancer patients was significantly increased compared with healthy volunteers [(27.69 ± 11.66) μg/ml vs (7.41 ± 5.2) μg/ml, P = 0.000]. Prostate cancer patients with distant metastasis experienced higher levels of exosomes when compared with patients without distant metastasis [(33.40 ± 10.45) μg/ml vs (18.17 ± 5.96) μg/ml, P<0.05].The Capacity of proliferation and secretion was positively associated with increase of exosomes.Number of pulmonary metastasized nodules in mice treated with exosomes were increased significantly compared with that in mice not receiving exosomes [(16.28 ± 6.94) vs (4.72 ± 2.51), P = 0.000]. Conclusion Capacity of cell proliferation,exosome secretion and tumor metastasis are positively associated with enhancement of exosomes.关键词
外泌体/前列腺癌/肿瘤进展/肿瘤转移Key words
exosomes/prostate cancer/cancer development/metastasis分类
医药卫生引用本文复制引用
吴维,江娟,吕磊,梁嘉前,孙莹,袁敬东,章传华,周高峰..外泌体在前列腺癌发展和转移中的作用[J].中国现代医学杂志,2018,28(6):38-44,7.基金项目
武汉市卫生和计划生育委员会科研项目(No:WX17C04) （No:WX17C04）
