中国现代医学杂志2018，Vol.28Issue(6)：45-49,5.DOI:10.3969/j.issn.1005-8982.2018.06.008
miRNA-138调控卵巢上皮癌细胞对顺铂敏感性的机制研究
Regulative role of miRNA-138 on platinum sensitivity of ovarian epithelial cancer cells
周宏博 1桂艺方2
作者信息
- 1. 牡丹江医学院红旗医院肿瘤科,黑龙江 牡丹江 157011
- 2. 牡丹江医学院红旗医院检验科,黑龙江 牡丹江 157011
- 折叠
摘要
Abstract
Objective To investigate the regulative effect of miRNA-138 on chemo-sensitivity of ovarian epithelial cancer to platinum and potential mechanism. Methods Totally 80 cases of patients with ovarian epithelial cancer admitted into Mudanjiang Medical College Hongqi Hospital from January 2014 to January 2017 were included in this study. Patients were divided into two groups: platinum-sensitive group and platinum-resistant group. Quantitative real-time PCR (RT-qPCR) and Western Blot were performed to detect the expression of miRNA-138 and SIRT1 in ovarian epithelial carcinoma. SKOV3 cell line were transfected with miRNA-138 analogs (miRNA-138 mimic group) and inhibitor (miRNA-138 inhibitor group). Expression of SIRT1 mRNA was measured by RT-qPCR. MTT assay was utilized for determination of sensitivity to platinum. SIRT1 mRNA 3'-UTR wild type and mutant type luciferase reporter plasmids were co-transfected with miRNA-138mimic, and double luciferase reporter system was used to analyze luciferase activity. Results The concentration of miRNA-138 in platinum-sensitive group was significantly higher than that in platinum-resistant group, while levels of SIRT1 was significantly decreased compared with platinum-resistant group (P < 0.05). Overexpression of miRNA-138 decreased the expression of SIRT1 and increased the sensitivity of tumor cells to platinum (P < 0.05), which was reversed by inhibition of miRNA-138 (P < 0.05). Luciferase activity was significantly reduced in group with transfection of SIRT1 mRNA 3'-UTR wild-type plasmids, while no significant changes in luciferase activity were observed in transfected SIRT1 mRNA 3'-UTR mutant plasmid (P > 0.05). Conclusion MiRNA-138 may regulate the sensitivity of ovarian epithelial cancer cells to platinum through SIRT1 activity, which can be a potential therapeutic target of ovarian epithelial cancer.关键词
卵巢上皮癌/miRNA-138/沉默信息调节因子2相关酶1/顺铂/敏感性Key words
ovarian epithelial carcinoma/miRNA-138/SIRT1/platinum chemosensitivity分类
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周宏博,桂艺方..miRNA-138调控卵巢上皮癌细胞对顺铂敏感性的机制研究[J].中国现代医学杂志,2018,28(6):45-49,5.
