中国现代医学杂志2018，Vol.28Issue(7)：1-6,6.DOI:10.3969/j.issn.1005-8982.2018.07.001
激活素相互作用蛋白5在急性肝损伤动物模型中的表达变化及意义
ActRIP5 expression and significance in animal model of acute liver injury*
摘要
Abstract
Objective To investigate the expressions and significance of activin and activin receptor-interacting protein 5 (ActRIP5) in the process of animal acute immune liver injury induced by concanavalin A (ConA). Methods ConA (15 mg/kg) was injected into C57BL/6 mice through tail vein. In different periods after administration of ConA, the changes of serum enzymes, and liver and spleen morphology and indexes were determined in the model mice, the expressions of activin and ActRIP 5 were detected by fluorescence quantitative PCR. Results The serum levels of ALT and AST in the liver injury model began to increase 4 h after ConA injection and reached the peak in 24 h, then gradually decreased, but did not yet return to the normal level in 96 h. The spleen index of the mice began to increase 2 h after administration and reached the peak after 24 h, and began to decline after 48 h, and returned to the normal level after 96 h. The liver index began to increase 24 h after administration and reached the maximum after 48 h, then began to decrease, and did not return to normal yet after 96 h. The morphological and pathological examination revealed that liver injury began to appear 4 h after administration and was most serious between 24 h and 48 h, and began to recover after 48 h, but did not return to normal yet after 96 h. Fluorescence quantitative PCR results showed that activin A began to decline 2 h after administration and reached the peak after 8 h, then began to decrease, and got to the normal level afeter 96 h; ActRIP5 began to rise 4 h after administration and reached the peak after 8 h, then began to decline, but did not return to the normal level after 96 h. Conclusions ConA can induce acute liver injury. The expressions of activin and ActRIP5 abnormally elevate during the progressive stage of liver injury, and decrease and return to normal during the recovery period of liver injury, suggesting that activin may participate in ConA-induced acute liver injury through the specific signal transduction protein ActPIR5.关键词
急性肝损伤/激活素/激活素相互作用蛋白5/刀豆蛋白AKey words
acute liver injury/activin/activin receptor-interacting protein 5/concanavalin A分类
医药卫生引用本文复制引用
徐元红,鞠宝玲,聂影,宋传芳,吴昆,庄彦华,张红军..激活素相互作用蛋白5在急性肝损伤动物模型中的表达变化及意义[J].中国现代医学杂志,2018,28(7):1-6,6.基金项目
国家自然科学基金(No:81270513) （No:81270513）
