中国现代医学杂志2018，Vol.28Issue(7)：7-11,5.DOI:10.3969/j.issn.1005-8982.2018.07.002
2型糖尿病小鼠不同时间点的病理生理变化
Pathological and physiological changes of type 2 diabetic mice at different time points*
摘要
Abstract
Objective To determine the time of pathological changes of type 2 diabetes mellitus mouse model by detecting serum insulin and the indexes of metabolism and cardiac function in type 2 diabetic mice at different time points. Methods Male 8-week C57BL/6 mice were used to establish the animal model of type 2 diabetes mellitus by high fat and low-dosage Streptozocin (STZ). At different time points after STZ injection, the fasting blood glucose in mouse tail artery was measured using the Roche glucometer. Serum insulin concentration was tested using mouse insulin ELISA kit. Small animal ultrasound was applied for detection of left ventricular function in the mice. Hematoxylin-eosin staining and Masson staining were used to observe the shape and size of the heart and myocardial fibrosis. Results In the experimental group, fasting blood glucose was significantly increased 1 week after STZ injection; glucose tolerance and insulin tolerance were impaired 2 weeks after STZ injection; at the 8th week, abnormal serum insulin and left ventricular diastolic dysfunction appeared and left ventricular hypertrophy and fibrosis were observed; at the 12th week, left ventricule was dilated, fibrosis was aggravated. Conclusions In the 2nd week after STZ injection, fasting blood glucose significantly increases; in the 8th week, serum insulin decreases, left ventricular hypertrophy and diastolic dysfunction appear; at th 12th week, there is left ventricular systolic dysfunction. This study provides an experimental basis for the study of the pathogenesis and treatment of type 2 diabetes mellitus.关键词
链脲佐菌素/高脂喂养/2型糖尿病/小鼠Key words
Streptozocin/high-fat feeding/type 2 diabetes mellitus/mouse分类
医药卫生引用本文复制引用
齐艳萍,田孝祥,刘丹,吴鹏,闫承慧,韩雅玲..2型糖尿病小鼠不同时间点的病理生理变化[J].中国现代医学杂志,2018,28(7):7-11,5.基金项目
国家自然科学基金(81670276) （81670276）
辽宁省自然科学基金(No:20170540965,20170540929) （No:20170540965,20170540929）
