中国现代医学杂志2018，Vol.28Issue(7)：12-18,7.DOI:10.3969/j.issn.1005-8982.2018.07.003
神经细胞分化中microRNA-134靶基因网络功能分析
Analysis of microRNA-134 target gene interaction network in neural progenitor-like cell differentiation*
摘要
Abstract
Objective To detect the expression of microRNA-134 at different time after induction and better investigate the molecular mechanism in the regulation of neural differentiation. Methods Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) were used to induce the differentiation of bone marrow derived mesenchymal stem cells (BMSCs) into neural progenitor-like cells (NPCs). BMSCs were divided into two groups:control group (without the growth factors) and induction group (added with EGF and bFGF). The morphological changes were observed and the neuronal specific markers were detected by qRT-PCR and immunohistochemical technique. The expression of microRNA-134 was determined by qRT-PCR. The target gene interaction network was analyzed using miRWalk, DAVID, STRING and Cytoscape softwares. Results MicroRNA-134 was up-regulated after EGF/bFGF-induced differentiation of rat BMSCs, similar to the expression of microRNA-134 in the rat fetal brain. Bioinformatics analysis revealed that some target genes of microRNA-134 were associated with neuron differentiation,such as CASP3,ITGB1,PAFAH1B1,PRKCA,EFNA2,NKX6-1,NRCAM,STXBP1,KCNIP2,ABI2, CNTN2,and FOXA1. Conclusions MicroRNA-134 promotes the differentiation of BMSCs into NPCs through target genes.关键词
MicroRNA-134/骨髓间充质干细胞/神经细胞/分化Key words
microRNA-134/BMSCs/neuron/differentiation分类
医药卫生引用本文复制引用
苏立宁,尹海峰,宋小青,魏会平..神经细胞分化中microRNA-134靶基因网络功能分析[J].中国现代医学杂志,2018,28(7):12-18,7.基金项目
河北北方学院重大项目(No:ZD201413) （No:ZD201413）
