中国现代医学杂志2018，Vol.28Issue(7)：19-22,4.DOI:10.3969/j.issn.1005-8982.2018.07.004
α7nAChR激动剂对大鼠移植肺缺血再灌注损伤的保护作用
Protective effect of α7nAChR against lung ischemia-reperfusion injury after transplantation in rats*
摘要
Abstract
Objective To investigate the protective effect and possible mechanism of α7nAChR agonist (PNU 282987) on lung ischemia-reperfusion injury (IRI) by establishing rat allogeneic orthotopic left lung transplantation model. Methods The modified three-cuff anastomotic technique was used to establish the model of rat allogeneic orthotopic left lung transplantation. Healthy Sprague-Dawley male rats were randomly divided into 3 groups: sham operation group (group A), normal saline-treated IRI group (group B) and α7nAChR-treated group (group C). After the reperfusion for 4 h, the right pulmonary hilum was blocked for 5 min. The functions of the graft were checked by PaO2and PaCO2. Then the transplanted lung tissues were collected to measure the wet/dry ratio and the content of TNF-α and IL-6 by ELISA. Results Compared with the group A, PaO2decreased, while PaCO2, the wet/dry ratio and the content of TNF-α and IL-6 increased in the group B (P < 0.05). Compared with the group B, PaO2increased, simultaneously, the wet/dry ratio and the content of TNF-α and IL-6 decreased in the group C (P < 0.05); however, there was no statistical difference in PaCO2 between the two groups (P > 0.05). Conclusions α7nAChR agonist has protective effect on lung IRI after transplantation in rats and can improve lung functions by inhibiting inflammation.关键词
PNU282987/肺移植/缺血再灌注/大鼠Key words
PNU 282987/lung transplantation/ischemia and reperfusion/rat分类
医药卫生引用本文复制引用
赵正维,王海强,尹逊亮,张志培,李小飞,周勇安..α7nAChR激动剂对大鼠移植肺缺血再灌注损伤的保护作用[J].中国现代医学杂志,2018,28(7):19-22,4.基金项目
青年科学基金项目(No:81402411) （No:81402411）
