中国现代医学杂志2018，Vol.28Issue(7)：30-35,6.DOI:10.3969/j.issn.1005-8982.2018.07.006
FGF-1对TGF-β1诱导的H9c2心肌细胞凋亡与Caspase-3,Caspase-8和Caspase-9表达的关系研究
Effect of FGF-1 on caspase-3, caspase-8 and caspase-9 expressions and apoptosis of myocardial H9c2 cells induced by TGF-β1*
摘要
Abstract
Objective To observe the effect of acidic fibroblast growth factor (FGF-1) on the apoptosis of myocardial H9c2 cells induced by transforming growth factor-β1 (TGF-β1) and the expressions of cysteinyl aspartate specific proteinase-3 (caspase-3), caspase-8 and caspase-9 and to discuss its possible mechanisms. Methods H9c2 cells were cultured and randomly divided into 5 groups, i.e. model group, Heparin group, FGF-1 group, FGF-1/Heparin group and normal control group. The survival rate of H9c2 cells was measured by 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The apoptotic index of H9c2 cells was observed by TUNEL staining. The expression levels of caspase-3, caspase-8 and caspase-9 were detected by Western blot. Results Compared with the normal control group, the survival rate of H9c2 cells was significantly decreased in the model group (P < 0.05). Compared with the model group, the survival rate of H9c2 cells was significantly increased in the FGF-1 group and the FGF-1/Heparin group (P < 0.05). Compared with the normal control group, the apoptosis index and the expressions of caspase-3, caspase-8 and caspase-9 of H9c2 cells in the model group were significantly increased (P < 0.05). Compared with the model group, the apoptosis index and the expressions of caspase-3, caspase-8 and caspase-9 of H9c2 cells were significantly decreased in theFGF-1 group and the FGF-1/Heparin group (P < 0.05). Conclusions FGF-1 could obviously inhibit the apoptosis of H9c2 cells induced by TGF-β1 and its possible mechanism may be related to decrease of the expression level of caspase-3, caspase-8 and caspase-9.关键词
酸性成纤维细胞生长因子/H9c2心肌细胞/半胱氨酸蛋白酶-3/半胱氨酸蛋白酶-8/半胱氨酸蛋白酶-9Key words
acidic fibroblast growth factor/H9c2 cardiomyocyte/cysteinyl aspartate specific proteinase-3/cysteinyl aspartate specific proteinase-8/cysteinyl aspartate specific proteinase-9分类
医药卫生引用本文复制引用
李厚忠,郭金兴,张胜强,张羽飞..FGF-1对TGF-β1诱导的H9c2心肌细胞凋亡与Caspase-3,Caspase-8和Caspase-9表达的关系研究[J].中国现代医学杂志,2018,28(7):30-35,6.基金项目
黑龙江省卫生计生委科研课题(NO:2014-215) （NO:2014-215）
牡丹江医学院科学技术研究项目(NO:ZS201518) （NO:ZS201518）
黑龙江省大学生创新创业训练计划项目(NO:201410229021) （NO:201410229021）
