中国畜牧兽医2017，Vol.44Issue(11)：3113-3120,8.DOI:10.16431/j.cnki.1671-7236.2017.11.001
不同生物型BVDV感染宿主细胞后差异基因分析
Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus
摘要
Abstract
In order to further study the problem of persistent infection and investigate the molecular mechanism of transformation between different biotypes of bovine viral diarrhea virus (BVDV),cytopathic BVDV (CP BVDV) and non cytopathic BVDV (NCP BVDV) were used to infect MDBK host cell and the cells were observed and collected per 12 h from 12 to 72 h,and at the same time the transcriptome analysis were used to analyse the 24 and 48 h MDBK cells.The results showed that at 24 h,compared to MDBK cell infected by NCP BVDV,there were 2849 up-regulated and 3347 down-regulated differential genes,while 2933 up-regulated and 2831 down-regulated differential genes at 48 h in MDBK cell infected by CP BVDV.The results of GO function classification statistics showed that the molecular function of differential gene mainly involved in catalytic activity,binding activity,enzyme regulator activity,molecular transduction activity and protein binding transcription factor activity.Pathway saliency map showed that the genes involved in autophagy,apoptosis,signal transduction related expression of immunoregulatory factor were preliminary screened.The found of differential gene laid the foundation to further reveal the molecular mechanism of viral pathogenesis,also provided molecular targets for the development of new anti BVDV preparations.关键词
致细胞病变型BVDV/非致细胞病变型BVDV/差异基因/GO功能分析/Pathway显著性分析Key words
cytopathogenic BVDV (CP BVDV)/non cytopathic BVDV (NCP BVDV)/differential gene/GO function analysis/Pathway analysis分类
农业科技引用本文复制引用
程凯慧,朱彤,侯佩莉,王洪梅,苗自利,张亮,解晓莉,杨宏军,何洪彬..不同生物型BVDV感染宿主细胞后差异基因分析[J].中国畜牧兽医,2017,44(11):3113-3120,8.基金项目
十三五国家重点研发计划(2016YFD0500904) （2016YFD0500904）
山东省优秀中青年科学家科研奖励基金(BS2014SW008) （BS2014SW008）
现代农业(奶牛)产业技术体系科学家岗位(CARS-36) （奶牛）
山东省农科院青年基金(2014QNM17) （2014QNM17）
山东省自然基金(ZR2016CP09) （ZR2016CP09）
山东省农业科学院农业科技创新工程(CXGC2016B14、CXGC2016A10) （CXGC2016B14、CXGC2016A10）
