中国畜牧兽医2017，Vol.44Issue(11)：3121-3129,9.DOI:10.16431/j.cnki.1671-7236.2017.11.002
利用16S rDNA扩增子测序技术分析不同品种猪盲肠微生物菌落多样性
Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16S rDNA Tag
摘要
Abstract
To study the diversity and composition of the bacterial community from cecum samples from Yacha pig,Qingyu pig and Wujin pig,the investigation was designed to reveal mechanism of roughage resistance.Illumina amplicon sequencing of 16S rDNA Tag was used to analyze the cecum microbial diversity of Yacha pig,Qingyu pig and Wujin pig at 90 kg liveweight.The resuits indicated that the core flora of the three breeds were Bacteroidetes and Firmicutes at the phylum level and Prevotella and Bacteroides at the genus level.Meanwhile bacteria associated with cellulose decomposition were found in all three breeds,in which the number of Fibrobacter and Clostridium in Yacha pig were significantly higher than that in Qingyu pig and Wujin pig (P＜0.05),and the number of Prevotella in Yacha pig was significantly higher than that in Wujin pig (P＜0.05).The number of Bacteroides in Qingyu pig was significantly higher than that in Yacha pig (P＜0.05).The number of Spirochaetes in Qingyu pig was significantly higher than that in Wujin pig (P＜0.05).It could conclud that the composition of the cecum bacteria community was similar in above pig breeds,but there were significant differences in distribution and quantity.According to analysis of microbial related to cellulose digestibility,Yacha pig was the strongest among the three breeds,followed by the Qingyu pig and Wujin pig.关键词
丫杈猪/青裕猪/乌金猪/盲肠/微生物多样性/纤维素消化能力Key words
Yacha pig/Qingyu pig/Wujin pig/cecum/microbial diversity/cellulose digest ability分类
农业科技引用本文复制引用
康润敏,李瑶,吕学斌,姬高升,应三成,曾凯,李琰,殷明郁..利用16S rDNA扩增子测序技术分析不同品种猪盲肠微生物菌落多样性[J].中国畜牧兽医,2017,44(11):3121-3129,9.基金项目
四川公益性科研院所基本科研项目(SASA2015A03) （SASA2015A03）
四川省科技支撑计划(2016NYZ0042、2014NZ0092、16ZC2850) （2016NYZ0042、2014NZ0092、16ZC2850）
国家生猪产业技术体系(CARS-36) （CARS-36）
四川省财政运行专项(SASA2014CZYX001) （SASA2014CZYX001）
