中国畜牧兽医2017，Vol.44Issue(11)：3143-3148,6.DOI:10.16431/j.cnki.1671-7236.2017.11.005
体内、外卵母细胞对猪体细胞克隆胚胎发育潜力的影响
Effect of Oocytes in vivo and in vitro on the Developmental Potential of Porcine Somatic Cell Cloned Embryos
摘要
Abstract
To investigate the impact of porcine oocytes in vivo and in vitro maturation (IVM) on the development of porcine somatic cell cloned embryos,the somatic cell cloned embryos cultured in vitro and the sows were treated with hormones to collect mature oocytes in vivo,and the cleavage rate,blastocyst rate and embryo implantation were compared.The results showed that the average number of ovulation in PGC+PMSG+ HCG group was significantly higher than that of PGC+HCG,PMSG+HCG and the natural estrus groups (P＜0.05).The oocytes collected in vivo could be used for the construction,and the available oocytes rate reached more than 90％,and there was no significant difference among the four groups (P＞0.05),which indicated that groups treated by hormone could obtain more available oocytes and the quality of oocytes was not significant different.In vivo and in vitro matured oocytes were used as nuclear transfer embryos of recombinant receptor,the fusion efficiency (80.31％ and 79.29％) and cleavage rate (90.40％ and 86.51 ％) were not significant different (P＞0.05),but the proportion of in vivo matured oocytes cloned embryos developed into the blastocyst stage was significantly higher (P＜0.05).The reconstructed embryos made from in vivo and in vitro matured oocytes were transplanted into surrogate sows (transferred 30 or 60 embryos),10 piglets were born in in vivo maturation of cloned embryo transfer group,while there was no implantation in in vitro maturation of cloned embryo transfer group.The results showed that high quality oocytes obtained by superovulation could significantly increase the blastocyst rate of embryos,reduce the number of embryos transferred and improve the pregnancy rate of surrogate sows.关键词
卵母细胞/体细胞核移植/胚胎/猪Key words
oocytes/somatic cell nuclear transfer/embryos/sow分类
生物科学引用本文复制引用
华再东,郭帅,肖红卫,任红艳,张立苹,葛耀文..体内、外卵母细胞对猪体细胞克隆胚胎发育潜力的影响[J].中国畜牧兽医,2017,44(11):3143-3148,6.基金项目
国家转基因生物新品种培育重大专项(2016ZX08006002-006、2016ZX08006001-005、2016ZX08010003-006) （2016ZX08006002-006、2016ZX08006001-005、2016ZX08010003-006）
湖北省农业科技创新中心(2016-620-000-001-027) （2016-620-000-001-027）
