中国畜牧兽医2017，Vol.44Issue(11)：3163-3169,7.DOI:10.16431/j.cnki.1671-7236.2017.11.009
牦牛血液氯化血红素的分离提纯及工艺优化研究
Study on the Separation and Purification of Hemin from Yak Blood and Its Optimization
摘要
Abstract
The study was conducted to separate and purify the hemin from yak blood and the optimal process of preparing the hemin was confirmed using blood meal,glacial acetic acid and sodium acetate methods.The purity and the yield of the extracted hemin in different methods were compared.The results showed that the purity of hemin in yak blood was 96％ and the yield was 7.38 g/L using blood meal method under the optimal process of 40 min for ultrasonic treatment,30 min for extraction,120 mL acid acetone and 9 mL sodium acetate.The purity of hemin was 80％ and the yield was 6.10 g/L when the amount of glacial acetic acid was 3 times as much as blood cells amount using the glacial acetic acid method.The purity of hemin was 90％ and the yield was 3.44 g/L when the amount of chloroform was 2 times as much as blood cells amount using sodium acetate method.The purity of the hemin from high to low was blood meal method,sodium acetate method and glacial acetic acid method,and that of the hemin yield was blood meal method,glacial acetic acid method and sodium acetate method by comparing the three preparation methods.So the blood meal method was more conducive to industrial production.关键词
牦牛血液/氯化血红素/牦牛血粉/醋酸钠法/冰醋酸法Key words
yak blood/hemin/yak blood meal/sodium acetate method/glacial acetic acid method分类
农业科技引用本文复制引用
杨生忠,韩学燕,李宗文,陈虎,任茜,曹效海..牦牛血液氯化血红素的分离提纯及工艺优化研究[J].中国畜牧兽医,2017,44(11):3163-3169,7.基金项目
科技厅科技支撑项目“青藏高原动物血液综合利用技术产业化” （）
