中国畜牧兽医2017，Vol.44Issue(11)：3179-3186,8.DOI:10.16431/j.cnki.1671-7236.2017.11.011
米曲霉0-8固态发酵条件优化及其发酵产物对肉仔鸡日粮养分消化的影响
Optimization of 0-8 Aspergillus oryzae Solid State Fermentation Conditions and the Effect of Fermented Product on Nutrient Digestion of Broiler
摘要
Abstract
The high-yield protease was obtained by screening high-yield protease Aspergillus oryzae and optimizing its solid-state fermentation conditions,and then the effect of fermented product on nutrient digestion in broiler was studied.After the screening and re-screening of 8 different Aspergillus oryzae spores,a new Aspergillus oryzae strain 0-8 with high acid protease was obtained.The fermentation conditions of acid protease were optimized by single factor and orthogonal test experiment.The results showed that the optimum temperature was 30 ℃,the most suitable fermentation time was 66 h,the initial water content was 47.40％,the inoculation quantity was 1.5 × 107 spores/mL,the content of bran was 80％,C/N was 1/3,the optimized vigor of acid protease reached 14 416.64 U/g,which was 157.02％ higher than that before optimization.500 mg/kg Aspergillus oryzae 0-8 fermented product was added in the broiler diet,and the mutrient digestibility of broiler were measured after 10 d of adaptation and 5 d of test period.The results of the nutrient digestion of broilers showed that feeding Aspergillus oryzae 0-8 fermentation increased the CP and energy digestibility of broiler in a certain degree compared to control group,but there's no significant difference (P＞0.05).Dietary supplementation of Aspergillus oryzae fermentation was beneficial to the production of broilers,but its additive dose or feeding pattern should be further studied.关键词
米曲霉/固态发酵/酸性蛋白酶/肉仔鸡Key words
Aspergillus oryzae/solid state fermentation/acidic protease/broiler分类
农业科技引用本文复制引用
马剑青,郭艳丽,张铁鹰..米曲霉0-8固态发酵条件优化及其发酵产物对肉仔鸡日粮养分消化的影响[J].中国畜牧兽医,2017,44(11):3179-3186,8.基金项目
中国农业科学院科技创新工程专项经费(ASTIP-IAS08) （ASTIP-IAS08）
“十二五”农村领域国家科技计划“生态环保饲料生产关键技术研发与集成示范”(2011BAD26803) （2011BAD26803）
