中国畜牧兽医2017，Vol.44Issue(11)：3334-3339,6.DOI:10.16431/j.cnki.1671-7236.2017.11.032
鸭坦布苏病毒可视化RT-LAMP快速检测方法的建立与初步应用
Establishment and Application of Visual RT-LAMP for Rapid Detection of Duck Tembusu Virus
摘要
Abstract
To realize the rapid detection of duck tembusu virus (DTMUV),a set of specific primers was designed according to the highly conserved sequence of DTMUV E protein gene published in GenBank.A sensitive and convenient RT-LAMP amplification method was established by optimizing the concentrations of each component,reaction time and temperature in the reaction system.The results showed that the influence of betaine on the reaction system was not obvious under the optimal conditions.This method was 100 times more sensitive than the conventional RT-PCR.The RT-LAMP method specifically amplified tembusu virus but not other common avian viruses.The results could be differentiated by naked eyes.74 clinic suspicious samples were tested and 65 of them were positive.These results suggested that the RT-LAMP assay could be used as a method for the diagnosis and detection of clinical cases,and molecular epidemiological investigation of duck tembusu virus disease.关键词
鸭坦布苏病毒(DTMUV)/环介导等温扩增/可视化/快速检测Key words
duck tembusu virus (DTMUV)/RT-LAMP/visualization/rapid detection分类
农业科技引用本文复制引用
吴植,夏文龙,蔡树东,郭长明,袁维峰,王永娟..鸭坦布苏病毒可视化RT-LAMP快速检测方法的建立与初步应用[J].中国畜牧兽医,2017,44(11):3334-3339,6.基金项目
国家重点研发计划(2017YFD0500905) （2017YFD0500905）
江苏省教育厅自然基金面上项目(16KJB230004) （16KJB230004）
江苏省“六大人才”高峰第十二批培养对象支助项目(NY023) （NY023）
