中国畜牧兽医2017，Vol.44Issue(11)：3351-3357,7.DOI:10.16431/j.cnki.1671-7236.2017.11.034
牛奶中雌二醇胶体金试纸条快速检测技术研究
Study on Colloidal Gold Immunochromatographic Assay for Rapid Detection of Estradiol in Milk
摘要
Abstract
In order to develop a sensitive and specific method for the detection of estradiol residues (E2) in milk,a colloidal gold immunochromatographic method was established.Colloidal gold particles were prepared by trisodium citrate reduction method and labeled with rabbit polyclonal antibodies (PcAb) by physical adsorption method.After optimization of reaction conditions such as the amount of coating antigen and labeled antibody to assemble test strip,the sensitivity,specificity,repeatability and accelerated preservation of the test strip were determined.The estrogen analogue cross reaction and milk matrix interference reaction were also measured.The results showed that the optimized concentration of E2-OVA antigen was 2 mg/mL and the optimized antibody concentration was 20 μg/mL,visual detection limits of E2 was 10 μg/L in PBS within 10 min.The cross reaction rate of this method with estriol was 40 ％,there were no negligible crossreactivities with other estrogen compounds including estradiol valerate,estradiol benzoate,estrone,diethylstilbestrol,quinestrol,ethinyloestradiol and nonylphenol.Milk samples only needed two times diluted before analysis,and the results could judge by naked eye after 10 min with cut-off of 20 μg/L.The results demonstrated that the developed method was suitable for rapid on-site screening of E2 residues in milk samples.关键词
雌二醇/胶体金免疫层析/快速检测/牛奶Key words
estradiol (E2)/colloidal gold immunochromatography assay/rapid detection/milk分类
农业科技引用本文复制引用
白宇,张井,胡景炎,许永鹏,王树明..牛奶中雌二醇胶体金试纸条快速检测技术研究[J].中国畜牧兽医,2017,44(11):3351-3357,7.基金项目
浙江省公益性技术应用研究计划项目(2014C32046、2017C37087) （2014C32046、2017C37087）
温州市公益性科技项目(S20150026) （S20150026）
