中国药房2017，Vol.28Issue(32)：4499-4502,4.DOI:10.6039/j.issn.1001-0408.2017.32.09
高效液相色谱法测定人血浆中伏立康唑的浓度
Determination of Voriconazole in Human Plasma by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the concentration determination of voriconazole in human plasma.METHODS:Plasma samples were precipitated with acetonitrile.Using ketoconazole as internal standard,HPLC method was adopted.The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution (1 ∶ 1∶98,V/V/V,pH was about 4.0)-acetonitrile (40∶60,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 255 nm.The column temperature was 40 ℃,and sample size was 20 μL.RESULTS:The linear range of voriconazole was 0.2-20.0 μg/mL.The limits of quantification was 0.2 μg/mL,and the minimum detection limit was 0.03 μg/mL.RSDs of inter-day and intra-day were lower than 10％.The method recoveries were 92.06％-106.26％ (RSD＜5％,n=5),and extraction recoveries were 75.62 ％-90.59 ％ (RSD ＜ 5 ％,n=5).The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL (n =10).CONCLUSIONS:The method is simple,rapid,specific and can be used for drug monitoring of voriconazole.关键词
伏立康唑/血药浓度/高效液相色谱法Key words
Voriconazole/Plasma concentration/HPLC分类
医药卫生引用本文复制引用
李学娟,刘四喜,陈泽彬,魏红,杨卫国,温鹏强..高效液相色谱法测定人血浆中伏立康唑的浓度[J].中国药房,2017,28(32):4499-4502,4.基金项目
深圳市卫生计生系统科研项目(No.201402053) （No.201402053）
