中国药理学通报2018，Vol.34Issue(2)：181-185,5.DOI:10.3969/j.issn.1001-1978.2018.02.008
Calpain抑制剂ALLN对H2O2诱导的661W细胞损害的影响
Possible effect of N-acetyl-leu-leu-norleucinal, an inhibitor of Calpain, on H2O2-induced damage in 661W cells
摘要
Abstract
Aim To investigate the possible effect of N-acetyl-leu-leu-norleucinal (ALLN),an inhibitor of Calpain,on H2O2-induced damage in 661W cells.Methods The cellular survival of 661W treated with different doses of H2O2 without or with ALLN for 24 h was measured by MTT assay.The protein level of Calpain 1 and Calpain 2 was assessed by Western blot.Results Upon the H2O2 treatment at the concentrations of 50,100,500,1 000 μmol · L-1,the survival rate of 661W significantly decreased in a dose-dependent manner compared to that of the control group.Furthermore,the protein level of Calpain 1 and Calpain 2 showed an obviously time-dependent increase in 661W cells treated with 100 μmol · L-1 H2O2 for 12,18,24 h.Finally,the survival rate of 661W treated with ALLN and H2O2 was higher than that treated only with H2O2,and there was no difference in survival rate between ALLN groups (at the concentrations of 25,50,100,200 μmol · L-1) and control group.Conclusions Calpain is involved in the damage induced by H2O2.ALLN,the inhibitor of Calpain,attenuates the oxidative damage,which plays a promising protective role in photo-receptor cells under oxidative stress.关键词
ALLN/661W/H2O2/Calpain蛋白/光感受器细胞/视网膜色素变性Key words
N-acetyl-leu-leu-norleucinal/661W/H2O2/Calpain protein/photo-receptor cells/retinitis pigmentosa分类
医药卫生引用本文复制引用
方丽君,林颖彬,黄恩,黄天文..Calpain抑制剂ALLN对H2O2诱导的661W细胞损害的影响[J].中国药理学通报,2018,34(2):181-185,5.基金项目
国家自然科学基金资助项目(No 81100812) （No 81100812）
福建省自然科学基金资助项目(No 2017J01292) （No 2017J01292）
