中国药理学通报2018，Vol.34Issue(2)：197-201,5.DOI:10.3969/j.issn.1001-1978.2018.02.011
海蓬子皂苷甲通过mTOR信号通路诱导MCF7细胞发生自噬
Bigelovii A induced autophagy in MCF7 cells by inhibiting mTOR signal pathway
摘要
Abstract
Aim To investigate the effects of Bigelovii A on autophagy and its mechanism.Methods Fluorescence microscope,flow cytometry and Western blot were employed to analyze autophagy.Western blot was used to detect the protein expressions of mTOR pathway.MTT colorimetry was used to assay cell viability after treatment with 3-MA and Bigelovii A or Bigelovii A alone.Results Bigelovii A-treated MCF7 cells displayed a dramatic increase in the number of MDC-labeled vesicles and the expressions of LC3-Ⅱ,indicating cell autophagy.Ⅰt was proved that in MCF7 cells,Bigelovii A inhibited mTOR signaling by decreasing Akt and p-ERK.Consistently,Bigelovii A decreased phosphorylation levels of mTOR,p70S6K (Ser371,Thr389) and 4EBP1 proteins.Inhibiting Bigelovii Ainduced autophagy with the autophagy inhibitor 3-methyladenine significantly decreased cell viability,which suggested that Bigelovii A-induced autophagy played a pro-survival role.Conclusion Bigelovii A is likely to induce autophagy through inhibiting mTOR pathway.关键词
海蓬子皂苷甲/MCF7细胞/细胞自噬/mTOR/Akt/ERKKey words
Bigelovii A/MCF7 cells/autophagy/mTOR/Akt/ERK分类
医药卫生引用本文复制引用
管福琴,赵友谊,冯煦,单宇,黄真真,王奇志,陈雨,印敏,刘飞,徐曙,王鸣..海蓬子皂苷甲通过mTOR信号通路诱导MCF7细胞发生自噬[J].中国药理学通报,2018,34(2):197-201,5.基金项目
国家自然科学基金资助项目(No 81402829) （No 81402829）
海洋公益性行业科研专项经费资助(No 201505023) （No 201505023）
江苏省自然科学基金资助项目(No BK20131338) （No BK20131338）
