中国药理学通报2018，Vol.34Issue(2)：225-231,7.DOI:10.3969/j.issn.1001-1978.2018.02.016
抗氧化肽AOP1对小鼠皮肤烫伤创面愈合修复的影响
Effect of antioxidant peptide AOP1 on healing repair of skin burns in mice
摘要
Abstract
Aim To explore the effect and mechanism of antioxidant peptide AOP1 on repair of skin burn wound healing in mice.Methods Fluorescence probe DCFH-DA was used to detect the changes of intracellular reactive oxygen species (ROS).Cell proliferation and migration assay were used to detect AOP1 toxicity and its effect on wound healing.Moreover,the skin scald wound was made on the shaved dorsum of the anesthetized mice with a 1.0 cm diameter brass cylinder heated in a water bath at 80 ℃ for 2 min and pressed against the rat skin for 10 s.The effects of AOP1 on the healing of skin burns were observed by HE and Masson staining and the contents of MDA and the activity of SOD in skin tissues were measured.Results The antioxidant peptide AOP1 could significantly reduce the number of ROS in HaCaT and L929 cells,and promote cell migration and proliferation.Compared with the untreated group,the skin healing time of AOP1 group was short,the healing rate was high,the area of scab was small,and inflammation and the content of MDA in burned tissue significantly decreased.The effect of AOP1 on healing of burn wound healing was also confirmed by HE and Masson staining.Conclusion It is suggested that the antioxidant peptide AOP1 with natural activity may promote the healing of skin burns by reducing the oxidative stress caused by burns.关键词
抗氧化肽/AOP1/皮肤烫伤/创面修复/氧化应激/炎症反应Key words
antioxidant peptide/AOP1/skin burns/wound repair/oxidative stress/inflammation分类
医药卫生引用本文复制引用
秦迪,张慧杰,杨希营,陈艳通,林维平,李文辉,孙同毅,高媛媛..抗氧化肽AOP1对小鼠皮肤烫伤创面愈合修复的影响[J].中国药理学通报,2018,34(2):225-231,7.基金项目
国家自然科学基金资助项目(No 31600775) （No 31600775）
山东省自然科学基金资助项目(No ZR2015HL056,ZR2016HM76) （No ZR2015HL056,ZR2016HM76）
山东省高等学校科技计划项目(No J15LM10) （No J15LM10）
山东省医药卫生科技发展计划项目(No 2015WS0058,2015WS0059) （No 2015WS0058,2015WS0059）
潍坊医学院教师公派教师国内访学项目经费资助 （）
