中国药理学通报2018，Vol.34Issue(2)：232-236,5.DOI:10.3969/j.issn.1001-1978.2018.02.017
ERK信号蛋白在吗啡预处理减轻大鼠全心缺血/再灌注损伤中的作用
Role of ERK signaling protein in morphine preconditioning reducing global ischemia-reperfusion injury in isolated rat hearts
摘要
Abstract
Aim To observe the role of ERK signaling protein in morphine preconditioning reducing global ischemia-reperfusion injury in isolated rat hearts.Methods Adult male Sprague-Dawley rats were distributed into six groups (n =10 for each) using a random number table:control group (CON),ischemia-reperfusion group (I/R),ischemia preconditioning group (IPC),morphine preconditioning group at the concentration of 1 μmol · L-1 (MPC),ERK inhibitor PD98059 + MPC (MPD),and group of ERK inhibitor-PD98059 (PD).The isolated rat hearts were treated on a Langendorff perfusion apparatus system.The coronary effluent was collected at 15 min of equilibration (baseline),5 and 10 min of reperfusion for detection of the activity of LDH.Meanwhile,a water-filled balloon was inserted into the left ventricular for continuous LVDP measurement.The IS and AAR and IS/AAR ratios were observed by TTC.Western blot was used to examine the level of phosphorylated ERK in myocardium.Results As compared with the I/R group,MPC significantly decreased IS and IS/AAR ratio as well as LDH activities at 5 min and 10 min of reperfusion,but improved the LVDP at the end of reperfusion.Moreover,the phosphorylation level of ERK in myocardium was up-regulated by MPC.However,ERK inhibitor PD98059 could block the protective effects of MPC,as indicated by the increased IS and IS/AAR ratio,elevated LDH activity at the reperfusion of 5 and 10 min,and the suppressed LVDP at the end of reperfusion.Furthermore,the MPC-induced phosphorylation of ERK was also reversed by PD98059.Conclusion Morphine preconditioning may confer cardio-protection against the global ischemia-reperfusion injury in rat hearts through enhancing the phosphorylation of ERK.关键词
细胞外信号调节激酶/吗啡预处理/阿片/受体/全心缺血/再灌注损伤/心肌保护Key words
extracellular signal regulated kinase/morphine preconditioning/opioid/receptor/global ischemia-reperfusion injury/cardio-protection分类
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黄俊,杨婉,金世云,产进中,刘中,张野,何淑芳..ERK信号蛋白在吗啡预处理减轻大鼠全心缺血/再灌注损伤中的作用[J].中国药理学通报,2018,34(2):232-236,5.基金项目
国家自然科学基金资助项目(No 81200171) （No 81200171）
安徽省高校自然科学研究项目重点项目(No KJ2017A172) （No KJ2017A172）
