中国药理学通报2018，Vol.34Issue(2)：237-243,7.DOI:10.3969/j.issn.1001-1978.2018.02.018
蛇床子素对髓核致炎神经根痛大鼠脊髓背角p38MAPK表达的影响
Effect of osthole on p38 MAPK signaling related pathway of rat model with inflammatory radicular pain induce by nucleus pulposus in the spinal horn
摘要
Abstract
Aim To observe the effect of epidurally application of osthole on the model of nucleus pulposusinduced inflammatory radicular pain and the expression of p38 MAPK signaling related pathway in the spinal dorsal horn of rats.Methods The model of radicular pain was generated by putting nucleus pulposus to the L5 dorsal root ganglion (DRG).50％ MWT was measured using Von Frey filaments to calculate mechanical pain threshold before and after operation.50 μL of 20 g · L-1 osthole was administered epidurally in group Ost and 50 μL of 100 mL · L-1 DMSO in group DMSO at postoperative day (POD).The expression of phosphorylated p38 (p-p38),IL-18 and IL-18R in the lumbar spinal dorsal horn was detected by Western blot.IL-18 mRNA was assessed by real-time PCR.Results The mechanical pain threshold significantly decreased after operation (P ＜ 0.05),while the expression of protein p-p38 MAPK,IL-18,IL-18R and IL-18 mRNA was significantly different.Compared with DMSO group,50％ MWT was significantly increased and accompanied with the decrease of protein p-p38,IL-18,IL-lgR and IL-18 mRNA in Ost group after drug administration (P ＜ 0.05).The correlation analysis between protein concentration of p38 MAPK and IL-18 mRNA showed that the Spearman correlation coefficient was 0.9 (P ＜ 0.05).Conclusion p-p38 and IL-18 of spinal dorsal horn participate in the rat model with inflammatory radicular pain induced by nucleus pulposus,and IL-18R plays a role in maintenance of the pain.Osthole administered epidurally in the early stage of pain could alleviate the pain for a long time,which may be related with inhibiting p38 MAPK signaling related pathways.关键词
蛇床子素/脊髓背角/p38 MAPK信号通路/IL-18/IL-18R/神经根炎性痛Key words
osthole/spinal dorsal horn/p38 MAPK signaling pathway/IL-18/IL-18R/inflammatory radicular pain分类
医药卫生引用本文复制引用
张嘉明,易增兴,林世清,王益敏,蔡哲,魏明,孙来保,邹学农..蛇床子素对髓核致炎神经根痛大鼠脊髓背角p38MAPK表达的影响[J].中国药理学通报,2018,34(2):237-243,7.基金项目
国家自然科学基金重点项目(No 31430030) （No 31430030）
惠州市大亚湾科技计划项目(No 2014A01015) （No 2014A01015）
