中国中医药信息杂志2018，Vol.25Issue(3)：81-85,5.DOI:10.3969/j.issn.1005-5304.2018.03.018
HepG2.2.15细胞对同时包载丁香苦苷和羟基酪醇纳米粒的摄取机制研究
Study on Cellular Uptake Mechanism of HepG2.2.15 Cells to Nanoparticles Co-loaded with Syringopicroside and Hydroxytyrosol
摘要
Abstract
Objective To investigate the uptake mechanism of HepG2.2.15 cells to the nanoparticles co-loaded with syringopicroside and hydroxytyrosol (SH-NPs). Methods The nanoparticles were prepared by using a nanoprecipitation method with mPEG-PLGA as nano-carrier co-loaded with syringopicroside and hydroxytyrosol. The uptake mechanism of HepG2.2.15 cells to SH-NPs was studied by fluorescence microscopy and flow cytometry using fluoresceineisothiocyanate (FITC) as a fluorescent marker. Results With colchicine as the inhibitor, the incubation time ranged from 0.5 to 24 h, the percentage of positive cells increased from 1.9% to 56.4%; When the drug concentration was 125, 250 μg/mL and 500 μg/mL, the positive cell percentages were 4.9%, 3.4% and 3.9%. With chloroquine as the inhibitor; the incubation time ranged from 0.5 to 24 h, the percentage of positive cells increased from 7.4% to 55.4%; When the drug concentration was 125, 250 and 500 μg/mL, the percentage of positive cells was 19.5%, 22.5% and 27.6%. Conclusion Colchicine and chloroquine have an inhibitory effect on HepG2.2.15 cells uptake, and the uptake of SH-NPs in HepG2.2.15 cells was positively correlated with drug concentration and incubation time. It can be concluded that the uptake mechanism of HepG2.2.15 cells to SH-NPs was nonspecific adsorption endocytosis.关键词
丁香苦苷/羟基酪醇/聚乙二醇-聚乳酸乙醇酸共聚物纳米粒/HepG2.2.15细胞/摄取机制Key words
syringopicroside/hydroxytyrosol/mPEG-PLGA nanoparticles/HepG2.2.15 cells/uptake mechanism分类
医药卫生引用本文复制引用
管庆霞,李云行,吕邵娃,孙佳琳,张亮,封文静,王利萍,李永吉..HepG2.2.15细胞对同时包载丁香苦苷和羟基酪醇纳米粒的摄取机制研究[J].中国中医药信息杂志,2018,25(3):81-85,5.基金项目
国家自然科学基金面上项目(81274091) （81274091）
黑龙江省自然科学基金面上项目(H2016076) （H2016076）
黑龙江中医药中青年科技攻关项目(ZQG-039) （ZQG-039）
黑龙江省教育厅科学技术研究项目(12531624) （12531624）
哈尔滨市应用技术研究与开发项目(2017RAQXJ090) （2017RAQXJ090）
