肿瘤药学2018，Vol.8Issue(1)：12-15,48,5.DOI:10.3969/j.issn.2095-1264.2018.01.03
β-榄香烯通过抑制PI3K/AKT/mTOR信号通路诱导A549细胞发生保护性自噬研究
β-elemene Induced Autophagy of A549 Cells by Inhibiting PI3K/AKT/mTOR Signaling Pathway
摘要
Abstract
Objective To investigate whether β-elemene could induce cell autophagy by inhibiting PI3K / AKT / mTOR signaling path-way to play an anti-tumor role. Methods MTT method was used to detect proliferation rate of A549 cells after treated with β-elemene. Au-tophagy phenomenon of acridine orange staining was observed under fluorescence microscope. The protein level of LC3- II, PI3K, AKT and mTOR were examined by Western blot after A549 cells treated with β-elemene. Results β-elemene could inhibit the proliferation of A549 cells obviously, and the inhibitory effect was enhanced with the increase of concentration of β-elemene. The IC50value of β-elemene on A549 cells was 72.45 μg·mL-1. Acridine orange staining results showed no abnormality was observed after A549 cells treated with β-elemene of a low concentration (10 μg·mL-1or 50 μg·mL-1). While treated at a high concentration of 100 μg·mL-1, 200 μg·mL-1, 500 μg·mL-1, the A549 cells showed obvious changes such as gradually vacuolated cytoplasm, increased acidic vesicles and gradually condensed nucleus. With the increase of β-elemene concentration, the expression of autophagy-related protein LC3-Ⅱ was gradually increased, while the expressions of PI3K, AKT and mTOR were decreased gradually, in a dose-dependent manner. Conclusion β-elemene could induce au-tophagy of A549 cells in a dose-dependent manner by inhibiting PI3K / AKT / mTOR pathway.关键词
β-榄香烯/PI3K/AKT/mTOR/自噬/肿瘤增殖Key words
β-elemene/PI3K/AKT/mTOR/Autophagy/Tumor proliferation分类
医药卫生引用本文复制引用
刘静冰,刘雄伟,吴夏慧,步伟金,姜子瑜..β-榄香烯通过抑制PI3K/AKT/mTOR信号通路诱导A549细胞发生保护性自噬研究[J].肿瘤药学,2018,8(1):12-15,48,5.基金项目
国家自然科学青年基金(NSFC 81503306) （NSFC 81503306）
江苏省自然科学青年基金(BK20151045). （BK20151045）
